Resveratrol Induces Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Neuron-Like Cells

Stem Cells International
Li GuoDe-Pei Li

Abstract

Objective. Human umbilical cord mesenchymal stem cells (hUC-MSCs) potentially differentiate to various types of cells including neuron-like cells. The natural polyphenol resveratrol benefits patients with many diseases including ischemic brain injury. We hypothesize that resveratrol induces differentiation of hUC-MSCs into neuron-like cells. Methods. Flow cytometry was used to determine the surface antigens in different stage of hUC-MSCs (P2, P5, and P10). Nestin, neuron-specific enolase (NSE), and glial fibrillary acidic protein (GFAP) were detected by immunocytochemistry, Western blotting, and real time RT-PCT. The cultured hUC-MSCs were treated with resveratrol at different concentrations (0, 7.5, 15.0, and 30.0 mg/L). Nestin, GFAP, and NSE protein and mRNA were measured at posttreatment time points of 2 h, 4 h, 6 h, 12 h, and 24 h. Results. Neuron-like cells were found in hUC-MSCs treated by resveratrol at concentrations of 15.0 and 30.0 mg/L, but not in hUC-MSCs treated with vehicle and 7.5 mg/L resveratrol. Furthermore, immunocytochemical staining revealed that nestin and NSE immunoreactivities were positive in resveratrol-treated hUC-MSCs at concentrations of 15.0 and 30.0 mg/L. Resveratrol treatment significantly increa...Continue Reading

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Citations

Apr 13, 2019·Regenerative Medicine·Agnieszka Borkowska-KuczkowskaDariusz Boruczkowski
May 19, 2019·International Journal of Molecular Sciences·Dariusz BoruczkowskiIzabela Zdolińska-Malinowska
Apr 17, 2019·Experimental and Therapeutic Medicine·Chen WangYangang Wang
Oct 30, 2018·Journal of Cellular and Molecular Medicine·Katarzyna KornickaKrzysztof Marycz
Oct 19, 2019·Stem Cell Research & Therapy·Chenxia Hu, Lanjuan Li
Dec 16, 2020·Stem Cell Research & Therapy·Anupong SongsaadCharoensri Thonabulsombat

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Methods Mentioned

BETA
flow cytometry
PCR

Software Mentioned

Primer

Related Concepts

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