Resveratrol inhibits LPS-induced epithelial-mesenchymal transition in mouse melanoma model

Innate Immunity
Man-Chin ChenChe-Hsin Lee

Abstract

Epithelial to mesenchymal transition (EMT) has been linked to metastasis. Resveratrol exhibits potential antitumor activities; however, the inhibitory effects of resveratrol on the EMT of melanoma have not been demonstrated. Here, a new role for LPS in promoting EMT is described. LPS-induced EMT was identified by examining the markers of EMT. To assess the activation of NF-κB signal transduction pathway, we performed a reporter assay by using tumor cells transfected with the luciferase gene under the control of NF-κB response elements. The antitumor effects of resveratrol were evaluated in an experimental mouse metastasis tumor model. LPS increased N-cadherin and Snail expression and decreased zonula occludens-1 expression in a dose- and time-dependent manner. Meanwhile, LPS stimulated cell migration through activation of TLR4/NF-κB signal pathway. LPS-induced EMT is critical for inflammation-initiated metastasis. Nuclear translocation and transcriptional activity of p65 NF-κB, an important inducer of EMT, were inhibited by resveratrol. Resveratrol inhibited LPS-induced tumor migration and markers of EMT, significantly prolonged animal survival and reduced the tumor size. Thus, resveratrol plays an important role in the inhibit...Continue Reading

References

Aug 22, 2002·Nature Reviews. Cancer·Jean Paul Thiery
Oct 26, 2002·Cancer Cell·Alan N Houghton, David Polsky
Mar 1, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M A BirrellM G Belvisi
Jun 1, 2005·Cancer Letters·Cristina MartínezMiguel Alcaraz
Jun 17, 2005·Cancer Research·Bo HuangHuabao Xiong
Sep 20, 2005·Cancer Cell·Susan E MoodyLewis A Chodosh
Jul 7, 2007·Science·Seth Rakoff-Nahoum, Ruslan Medzhitov
Feb 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chao-Liang WuAi-Li Shiau
Dec 3, 2009·Journal of Immunotherapy·Che-Hsin LeeAi-Li Shiau

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Citations

Aug 8, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tawona N ChinembiriJeanetta du Plessis
Feb 24, 2016·International Journal of Molecular Sciences·Yu-Tzu TsaoChe-Hsin Lee
Sep 23, 2014·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Yun-Qian ZhangLai Jiang
Feb 6, 2015·Biochimica Et Biophysica Acta·Eun-Jung Park, John M Pezzuto
May 12, 2016·American Journal of Clinical Dermatology·Adilson CostaJack L Arbiser
Nov 12, 2016·Nutrients·Aline Renata PavanJean Leandro Dos Santos
Dec 22, 2016·Critical Reviews in Food Science and Nutrition·Abdur RaufMohammad S Mubarak
Jan 23, 2014·Environmental Toxicology·Yu-Jung ChengChe-Hsin Lee
May 20, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Wen-Wen DongLai Jiang
Aug 14, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jie ShengYao Xu
Nov 13, 2019·The International Journal of Biochemistry & Cell Biology·Farjana Yeasmin KhusbuHan-Chun Chen
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shifang TangLijin Zhao
Jan 8, 2022·Nutrition and Cancer·Asefeh Dahmardeh GhalehnoKhadijeh Jamialahmadi

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Methods Mentioned

BETA
protein assay
nuclear translocation

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