Resveratrol inhibits pulmonary fibrosis by regulating miR-21 through MAPK/AP-1 pathways

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Jing WangJuan Yu

Abstract

To explore the molecular mechanism of Res in regulation of pulmonary fibrosis (PF). Rats were injected with bleomycin (BLM) to establish a PF model and treated with resveratrol (Res) and/or miR-21 agomir. After 14 days, lung tissues were collected for Hematoxylin-eosin and Masson's staining, and real-time quantitative polymerase chain reaction and Western blot were performed to detect fibrosis-related protein expression and the activation of the TGF-β1/Smad pathway. In vitro, MRC-5 cells were pretreated with TGF-β1, Res, and/or miR-21 agomir. After 48 h, total soluble collagen was detected with a Sircol Soluble Collagen Assay. Subsequently, a miR-21 mimic was transfected into MRC-5 cells, and a luciferase reporter assay was employed to verify whether miR-21 targeted Smad7. Res reversed the increased levels of miR-21 induced by BLM and alleviated serious PF symptoms, but agomiR-21 treatment effectively impaired the above manifestations. In vivo, miR-21 inhibited the decreases of TGF-β1 and p-Smad2/3 that were induced by Res. In vitro, miR-21 significantly disrupted the positive effect of Res on TGF-β-induced collagen deposition, as well as the levels of Fn, α-SMA, p-Smad2, and Smad7. In addition, Smad7 was found to be a direct t...Continue Reading

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Citations

Jun 9, 2020·Toxicology Mechanisms and Methods·Mikhail D ChanyshevLyudmila F Gulyaeva
Oct 4, 2020·Naunyn-Schmiedeberg's Archives of Pharmacology·Shuang ZhaoLisha Wu
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Feb 7, 2021·BioFactors·Seyede Atefe HosseiniAmirhossein Sahebkar
Mar 12, 2021·Frontiers in Medicine·Kun Yang, Wenbin Dong
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Sep 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yegang HuWei Zhang
Dec 14, 2019·Current Medicinal Chemistry·Atefeh AmiriHamed Mirzaei

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