Resveratrol Modifies Lipid Composition of Two Cancer Cell Lines

BioMed Research International
Luciana GomesEliane Fialho

Abstract

Resveratrol (Resv) offers health benefits in cancer and has been reported to modulate important enzymes of lipid metabolism. Studies of its effects on lipid composition in different subtypes of breast-cancer cells are scarce. Thus, we investigated the alterations in phospholipids (PL), fatty acids (FA), and lipid metabolism enzymes in two breast-cancer cell lines after Resv treatment. MCF-7 and MDA-MB-231 cells were treated with 80 and 200 μM of Resv, respectively, for 24 hours. We analyzed PL with radiolabeled inorganic phosphate (32Pi) by thin-layer chromatography, FA by gas chromatography-mass spectrometry, and lipid metabolism enzymes (DGAT2, FAS, ρACCβ, pAMPKα, and AMPK) by Western blot. Resv treated MDA-MB-231 phospholipids showed a reduction in phosphatidylcholine (63%) and phosphatidylethanolamine (35%). We observed an increase in eicosapentaenoic acid (EPA) (73%) and docosahexaenoic acid (DHA) (65%) in MCF-7 cells after Resv treatment. Interestingly, the same treatment caused 50% and 90% increases in EPA and DHA, respectively, in MDA-MB-231 cells. In MCF-7 cells, Resv increased the expression of ρACCβ (3.3-fold) and AMPKα/ρAMPKα (1.5-fold) and in MDA-MB-231 cells it inhibited the expression of ρACCβ (111.8-fold) and AM...Continue Reading

References

Sep 17, 1999·Journal of Clinical Microbiology·B A Arthington-SkaggsC J Morrison
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Jan 29, 2003·The Journal of Nutritional Biochemistry·Hilda ChamrasJohn A. Glaspy
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Oct 18, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Francesca ScarlattiRiccardo Ghidoni
Mar 24, 2005·Molecular Nutrition & Food Research·Ludovic Le CorreD J Bernard-Gallon
Jul 27, 2005·The Journal of Nutritional Biochemistry·Paola Signorelli, Riccardo Ghidoni
Mar 6, 2009·European Journal of Clinical Investigation·G V Gnoni, G Paglialonga
Sep 4, 2010·Breast Cancer Research : BCR·Aleix PratCharles M Perou
Dec 21, 2010·Journal of Chromatography. a·Beate FuchsJürgen Schiller
Dec 29, 2010·Breast Cancer Research and Treatment·Puspa R PandeyKounosuke Watabe
Jan 19, 2011·Omics : a Journal of Integrative Biology·Walter JägerDenise Sonntag
May 6, 2011·The Journal of Nutritional Biochemistry·Arrate LasaMaría del Puy Portillo
Mar 15, 2012·Journal of Cellular Biochemistry·Fabiana CasanovaEliane Fialho
Jul 7, 2012·Journal of Cellular Physiology·M Luísa DóriaLuisa A Helguero
Aug 18, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Beata Olas, Holm Holmsen
Nov 16, 2012·PloS One·Danielly Cristiny Ferraz da CostaJerson L Silva
Nov 10, 2013·Disease Models & Mechanisms·Franziska BaenkeAlmut Schulze
Feb 26, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Socrates Villegas-ComonfortEduardo Perez Salazar
Oct 16, 2014·Biochimica Et Biophysica Acta·Sameer S Kulkarni, Carles Cantó
Jan 7, 2015·International Journal of Molecular Sciences·Chunfa Huang, Carl Freter
May 13, 2015·Biochimica Et Biophysica Acta·Samy A F Morad, Myles C Cabot
May 29, 2015·Protist·Aline dos Anjos XimenesGeorgia C Atella
Jan 26, 2016·Oncogenesis·S Beloribi-DjefafliaF Guillaumond
Oct 19, 2016·Journal of Biophotonics·Evangelia GavgiotakiIrene Athanassakis
Sep 16, 2017·Journal of Food and Drug Analysis·Hui-Yun TsaiYu-Kuo Chen

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Citations

Aug 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danielly C Ferraz da CostaEliane Fialho

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

GraphPad Prism

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