Resveratrol prevents palmitic-acid-induced cardiomyocyte contractile impairment

Canadian Journal of Physiology and Pharmacology
Xavier Lieben LouisThomas Netticadan

Abstract

Long-chain saturated fatty acids, especially palmitic acid (PA), contribute to cardiomyocyte lipotoxicity. This study tests the effects of PA on adult rat cardiomyocyte contractile function and proteins associated with calcium regulating cardiomyocyte contraction and relaxation. Adult rat cardiomyocytes were pretreated with resveratrol (Resv) and then treated with PA. For the reversal study, cardiomyocytes were incubated with PA prior to treatment with Resv. Cardiomyocyte contractility, ratio of rod- to round-shaped cardiomyocytes, and Hoechst staining were used to measure functional and morphological changes in cardiomyocytes. Protein expression of sarco-endoplasmic reticulum ATPase 2a (SERCA2a), native phospholamban (PLB) and phosphorylated PLB (pPLB ser16 and pPLB thr17), and troponin I (TnI) and phosphorylated TnI (pTnI) were measured. SERCA2a activity was also measured. Our results show that PA (200 μM) decreased the rate of cardiomyocyte relaxation, reduced the number of rod-shaped cardiomyocytes, and increased the number of cells with condensed nuclei; pre-treating cardiomyocytes with Resv significantly prevented these changes. Post-treatment with Resv did not reverse morphological changes induced by PA. Protein expressi...Continue Reading

References

Dec 1, 1990·Analytical Biochemistry·W S Simonides, C van Hardeveld
Apr 1, 1989·The Journal of Clinical Investigation·M D JensenJ M Miles
Jul 1, 1997·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Van BilsenG J Van der Vusse
Mar 30, 2001·The Journal of Biological Chemistry·L L ListenbergerJ E Schaffer
Mar 2, 2005·Progress in Cardiovascular Diseases·Michael S Parmacek, R John Solaro
Mar 17, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Marcel C G DanielsPieter P de Tombe
Nov 9, 2007·Annual Review of Physiology·Donald M Bers
May 30, 2008·American Journal of Physiology. Cell Physiology·H J GreenJ Ouyang
Feb 10, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Halvor K MørkWilliam E Louch
Aug 19, 2009·Journal of Lipid Research·P Christian Schulze
Dec 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·M SulaimanM Gupta
Aug 17, 2010·Current Atherosclerosis Reports·Patty W Siri-TarinoRonald M Krauss
Nov 3, 2010·Journal of Molecular and Cellular Cardiology·Asli F Ceylan-IsikJun Ren
Mar 19, 2011·Journal of the American College of Cardiology·Cesare RussoMarco R Di Tullio
Mar 23, 2011·Current Pharmaceutical Design·Janine PössUlrich Laufs
Jul 16, 2011·European Journal of Pharmacology·Sijo J ThandapillyThomas Netticadan
Dec 6, 2011·The Journal of Nutritional Biochemistry·Xavier L LouisThomas Netticadan
May 29, 2012·Archives of Biochemistry and Biophysics·A MovahedT Netticadan
Sep 6, 2012·European Journal of Clinical Nutrition·N FaghihniaR M Krauss
Jan 3, 2013·The Journal of Clinical Investigation·Andrew R Marks
Feb 14, 2013·Primary Care·Robert H Nelson
Mar 20, 2013·Current Heart Failure Reports·Konstantinos Drosatos, P Christian Schulze
Mar 20, 2013·Mediators of Inflammation·Débora EstadellaAline de Piano
Jun 5, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Sue D Pedersen
Feb 21, 2014·Expert Review of Cardiovascular Therapy·John R Ussher
Jun 7, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Audrey E McCalleyPeter Koulen
Aug 15, 2015·International Journal of Circumpolar Health·Sven O E EbbessonBarbara V Howard
Sep 12, 2015·European Journal of Nutrition·Hsiu-Ching HsuMing-Fong Chen
Sep 13, 2015·Biochemical and Biophysical Research Communications·Frank SpillmannSophie Van Linthout
Oct 17, 2016·Current Obesity Reports·Martin A AlpertBrian P Bostick
Jan 1, 2015·International Journal of Circumpolar Health·Sven O E EbbessonBarbara V Howard

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