Resveratrol protects Lactobacillus reuteri against H2 O2 - induced oxidative stress and stimulates antioxidant defenses through upregulation of the dhaT gene

Free Radical Biology & Medicine
Narciza O ArcanjoMario Estévez

Abstract

Understanding of the mechanisms implicated in the protective role of probiotic bacteria is of the utmost scientific interest. This study provides original insight into the genetic and molecular basis of the responses of Lactobacillus reuteri PL503 against hydrogen peroxide (H2O2)-induced oxidative stress. Six experimental groups were considered depending on the addition and concentration of H2O2 and resveratrol: 1. CONTROL (L. reuteri in MRS broth); 2. H2O2 (L. reuteri in MRS broth + 0.5 mM H2O2); 3. LRES (L. reuteri in MRS broth + 20 μM resveratrol); 4. HRES (L. reuteri in MRS broth + 100 μM resveratrol); 5. H2O2-LRES (L. reuteri in MRS broth + 0.5 mM H2O2 + 20 μM resveratrol); 6. H2O2-HRES (L. reuteri in MRS broth + 0.5 mM H2O2 + 100 μM resveratrol). Three replicates were incubated at 37 °C for 24 h in microaerophilic conditions sampled at 12, 16, 20 and 24 h. The NADH-dependent-oxidoreductase encoded by the dhaT gene is a plausible candidate to be strongly implicated in the antioxidant response of L. reuteri. Resveratrol (100 μM) is found to protect L. reuteri against protein carbonylation plausibly through various mechanisms including direct scavenging of reactive oxygen species (ROS), upregulation of the dhaT gene and prom...Continue Reading

References

Apr 1, 1997·Journal of Pediatric Gastroenterology and Nutrition·A V ShornikovaT Vesikari
Aug 15, 1997·The Journal of Biological Chemistry·B S Berlett, E R Stadtman
Jun 23, 2000·Annals of the New York Academy of Sciences·E R Stadtman, R L Levine
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 5, 2002·International Journal of Cancer. Journal International Du Cancer·Daniel GackowskiRyszard Olinski
May 7, 2003·Current Opinion in Microbiology·Kristian KvintThomas Nyström
Feb 1, 2005·Biochimica Et Biophysica Acta·Michael J Davies
Jul 23, 2005·Annals of the New York Academy of Sciences·Mitsugu AkagawaKyozo Suyama
Jul 16, 2010·Digestive Diseases and Sciences·Basileios G SpyropoulosChristos N Stoidis
Jul 14, 2012·Applied Microbiology and Biotechnology·Alberto AmarettiAlessandra Bordoni
Jul 16, 2013·The Journal of Nutritional Biochemistry·Fernando CardonaMaría Isabel Queipo-Ortuño
Jul 11, 2014·Redox Biology·Fiorella BiasiGiuseppe Poli
Sep 9, 2015·Journal of Agricultural and Food Chemistry·Sisse JongbergMario Estévez
Apr 30, 2016·Critical Reviews in Food Science and Nutrition·M Estévez, C Luna
Oct 23, 2016·Free Radical Biology & Medicine·Annabell PlauthSascha Sauer
Apr 20, 2017·Nature Reviews. Microbiology·Benjamin EzratyJean-François Collet
Mar 1, 2015·Comprehensive Reviews in Food Science and Food Safety·O P SoladoyeM Estévez

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