Resveratrol protects late endothelial progenitor cells from TNF-α-induced inflammatory damage by upregulating Krüppel-like factor-2.

Molecular Medicine Reports
Hairong ChuMin Cheng

Abstract

Cardiovascular risk factors can negatively influence late endothelial progenitor cell (EPCs) number and functions, thus EPCs biology is a clinical implications for cardiovascular diseases. The present study aimed to investigate the potential protective effects of resveratrol (RES) on tumor necrosis factor (TNF)‑α‑induced inflammatory damage in late endothelial progenitor cells (EPCs) and to elucidate the underlying mechanisms. Late EPCs at passages 3‑5 were pretreated with RES at a concentration of 20 µmol/l for 12 h and subsequently incubated with TNF‑α (10 ng/ml) for 24 h. The adhesion, migration, proliferation and vasculogenesis of EPCs were subsequently detected. Furthermore, the mRNA expression levels of intercellular adhesion molecule‑1 (ICAM‑1) and monocyte chemoattractant protein‑1 (MCP‑1) were measured by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). Nitric oxide (NO) levels in the supernatant were determined using a colorimetric assay kit. Additionally, the mRNA and protein expression of Krüppel‑like factor‑2 (KLF2) was determined by RT‑qPCR and western blot analysis, respectively. The results indicated that TNF‑α markedly inhibited the proliferation, adhesion, migration and vasculogenesis of...Continue Reading

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Methods Mentioned

BETA
density gradient centrifugation
Protein Assay

Software Mentioned

Pro Plus
SPSS
Image
GelPro Analyser

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