Resveratrol regulates blood pressure by enhancing AMPK signaling to downregulate a Rac1-derived NADPH oxidase in the central nervous system

Journal of Applied Physiology
Tung-Chen YehPei-Wen Cheng

Abstract

Resveratrol is a polyphenol with pleiotropic effects against oxidative damage that has been widely implicated in lowering hypertension risk. The purpose of this study was to determine whether improve nitric oxide (NO) release in the brain, either through the activation of AMP-activated protein kinase (AMPK) or reduced Ras-related C3 botulinum toxin substrate 1 (Rac1)-induced reactive oxygen species (ROS) generation, thereby reducing blood pressure (BP) in rats with fructose-induced hypertension. The rats were fed with 10% fructose or Crestor (rosuvastatin; 1.5 mg·kg-1·day-1) and resveratrol (10 mg·kg-1·day-1) treatment for 1 wk, then the systolic blood pressure of the rats was measured by tail-cuff method. Endogenous in vivo superoxide radical production in the nucleus tractus solitarii (NTS) was determined with dihydroethidium. Immunoblotting analyses were used to quantify protein expression levels. Oral resveratrol treatment for 1 wk decreased BP and increased NO production in the NTS of fructose-fed rats but not in the control Wistar-Kyoto rats. The effect of Crestor is opposite that of resveratrol. Fructose induced hypertension by inactivating AMPK, which in turn enhanced the generation of ROS and reduced manganese superoxi...Continue Reading

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Citations

Oct 23, 2018·Journal of Periodontal Research·Mônica Grazieli CorrêaSuzana Peres Pimentel
Mar 10, 2020·BioMed Research International·Brhane Teklebrhan AssefaMeles Tekie Gidey
Aug 23, 2020·Journal of Periodontology·Fabiano Ribeiro CiranoSuzana Peres Pimentel

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Methods Mentioned

BETA
fluorescence microscopy
protein
electrophoresis
ELISA
Assay
confocal microscopy
GTPase
transfection

Software Mentioned

AICAR
ARRIVE
Zeiss LSM Image
NIH Image densitometry

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