Resveratrol significantly improves the fertilisation capacity of bovine sex-sorted semen by inhibiting apoptosis and lipid peroxidation

Scientific Reports
Chong-Yang LiXue-Ming Zhao

Abstract

The aim of this study was to test the effects of five different concentrations (0, 10-3, 10-4, 10-5, and 10-6 M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10-3 M and 10-4 M Res groups were significantly lower than those of controls (P < 0.05); (2) the percentage of viable sperm, percentage of sperm with high Δψm, and the ATP content in 10-3 M and 10-4 M Res groups were significantly higher than those of controls (P < 0.05); (3) the percentage of viable sperm with acrosomal integrity, and the blastocyst percentage and quality of the 10-4 M Res group were significantly higher than those of controls (P < 0.05). In conclusion, 10-4 M Res supplementation in washing and fertilisation medium of sex-sorted bull sperm significantly decreased ROS, PS externalisation, and MDA, and protected mitochondrial function and acrosomal integrity, thereby increasing blastocyst...Continue Reading

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Citations

Jun 3, 2020·Zygote : the Biology of Gametes and Early Embryos·Linlin SunWenxian Zeng
Dec 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Michal DurackaEva Tvrda
Mar 4, 2020·Tropical Animal Health and Production·Yulin HeTaiyong Yu
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Aug 18, 2020·Domestic Animal Endocrinology·S Gimeno-MartosA Casao
Sep 25, 2020·Reproduction in Domestic Animals = Zuchthygiene·Tian-Yu FengJian-Hong Hu
Apr 11, 2021·Reproductive Biology and Endocrinology : RB&E·Dalin WangYanqiu Hu
Aug 19, 2021·Reproduction in Domestic Animals = Zuchthygiene·Ramanathan KasimanickamKamron Ratzburg

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Methods Mentioned

BETA
flow cytometry
MDA
Assay
confocal microscopy
PCR

Software Mentioned

Statistical Analysis System

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