Resveratrol stimulation induces interleukin-8 gene transcription via NF-κB

Pharmacological Research : the Official Journal of the Italian Pharmacological Society
Gerald ThielOliver G Rössler

Abstract

The polyphenol resveratrol activates stimulus-regulated transcription factors, including activator protein-1 (AP-1). As part of a search for resveratrol-regulated target genes we analyzed the gene encoding the chemokine interleukin-8 (IL-8) which is regulated by AP-1. Here, we show that treatment of HEK293 cells with resveratrol induced the expression of IL-8 and activated transcription of a chromatin-embedded IL-8 promoter-controlled reporter gene. Mutational analysis of the IL-8 promoter revealed that it was not the AP-1 binding site, but rather the NF-κB site that was essential to connect resveratrol stimulation with the transcriptional activation of the IL-8 gene. Thus, the NF-κB site of the IL-8 gene functions as resveratrol-responsive element. The analysis of an NF-κB-responsive reporter gene, controlled by the HIV-1 long terminal repeat (LTR), showed that resveratrol stimulation increased the transcriptional activity of NF-κB. These data were corroborated by an experiment showing that incubation of the cells with the NF-κB inhibitor JSH-23 attenuated resveratrol-induced activation of the IL-8 promoter and reduced the cellular NF-κB activity following stimulation of the cells with resveratrol. The protein kinase extracell...Continue Reading

Citations

Nov 21, 2019·International Journal of Molecular Medicine·Zhongrong GuoYuanliang Huang
Mar 1, 2019·International Journal of Molecular Sciences·Jung-Im NaKyung-Chan Park
Jan 8, 2020·Bioscience, Biotechnology, and Biochemistry·So YamamotoShoko Kobayashi
Aug 31, 2021·Journal of Agricultural and Food Chemistry·Yen-Chun KohMin-Hsiung Pan

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