Resveratrol synergistically augments anti-tumor effect of 5-FU in vitro and in vivo by increasing S-phase arrest and tumor apoptosis

Experimental Biology and Medicine
Jiening DunYongjian Zhang

Abstract

Many studies have shown that natural dietary agents, in combination with chemical agents, can improve the therapeutic response of cancers to chemotherapy and reduce the associated side-effects. In the present study, we investigated the therapeutic potential and mechanisms of anticancer effects for the combination of 5-fluorouracil (5-FU) and resveratrol (Res). In these studies, we employed the cancer cell lines TE-1 and A431 and an animal model of skin cancer. The presented results provide the first evidence that Res can enhance the anti-tumor potency of 5-FU by inducing S-phase arrest. The combination of Res and 5-FU demonstrates synergistic efficacy, causing tumor regression in a two-stage model of mouse skin carcinogenesis induced by DMBA and TPA. There was clear evidence of Res augmenting the growth inhibitory effect of 5-FU on the TE-1 and A431 cancer cells in vitro. In the in vivo studies, the tumor regression rate in the combination group increased significantly after four weeks of treatment (P < 0.01). The combination of 5-FU and Res significantly increased the percentage of apoptotic cells and the level of activated caspase-3, cleaved PARP and p53 proteins as well as increased the Bax/Bcl-2 ratio. In conclusion, the 5-...Continue Reading

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Citations

Oct 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Mª Del Mar Blanquer-RossellóAdamo Valle
Sep 14, 2017·Oxidative Medicine and Cellular Longevity·Lalita SubediSun Yeou Kim
Jun 23, 2020·Experimental Biology and Medicine·Zhiyong ShenWeixing Qu
Sep 26, 2017·Annals of the New York Academy of Sciences·Zhengdong JiangWei Yang
Feb 2, 2021·Experimental Dermatology·Alicia S OmbredaneGraziella A Joanitti
Sep 26, 2015·Bioscience, Biotechnology, and Biochemistry·Na HeChunlei Zhu
Apr 18, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohammad Kashif IqubalSanjula Baboota
May 31, 2021·Cancer Letters·Boxu RenBoon Cher Goh
Apr 20, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sonali GhoshManju Ray

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Methods Mentioned

BETA
flow cytometry
Protein Assay
fluorescence microscopy
xenograft

Software Mentioned

CellQuest

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