Resveratrol Treatment in Different Time-Attenuated Neuronal Apoptosis After Oxygen and Glucose Deprivation/Reoxygenation via Enhancing the Activation of Nrf-2 Signaling Pathway In Vitro

Cell Transplantation
Jun YangJohn Zhang

Abstract

Recent studies have indicated that resveratrol has protective effects against cerebral ischemia/reperfusion injury. However, the best therapeutic time for resveratrol treatment after acute ischemic stroke remains unknown. We aim to investigate whether resveratrol, administrated at different times after neuronal oxygen and glucose deprivation/reoxygenation (OGD/R) reduced neuronal injury in vitro. There were six experimental groups: normal, model, resveratrol pretreatment, resveratrol post-treatment, resveratrol OGD-treatment, and resveratrol whole-processing group. We found that resveratrol in a concentration-dependent manner decreased the activity of lactate dehydrogenase (LDH) and increased the activity of superoxide dismutase (SOD). Moreover, resveratrol, administrated at different times, increased neuronal viability, reduced neuronal apoptosis, upregulated the protein expressions of Nuclear factor erythroid 2-related factor 2 (Nrf-2), NAD(P)H: quinone oxidoreductase 1 (NQO-1), heme oxygenase 1 (HO-1), and Bcl-2, downregulated the protein expression of Caspase-3, and promoted Nrf-2 to transfer into the nuclei from the cytoplasm. The most effective treatment group was the whole-processing treatment group. These results sugges...Continue Reading

References

Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·K D Poss, S Tonegawa
Mar 21, 2001·Apoptosis : an International Journal on Programmed Cell Death·H U SimonF Levi-Schaffer
May 19, 2001·Neuroepidemiology·K R EvensonD L Morris
Sep 19, 2003·Biochemical and Biophysical Research Communications·Stephen S LeonardXianglin Shi
Dec 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Marcus J CalkinsJeffrey A Johnson
Jul 4, 2006·Free Radical Biology & Medicine·Jack van HorssenHelga E de Vries
Jun 30, 2007·Journal of Vascular Surgery·Shen-Kou TsaiShiang-Suo Huang
Oct 25, 2007·Biochemical Society Transactions·C Alarcón de la Lastra, I Villegas
Apr 17, 2008·Cell Cycle·Kuzhuvelil B Harikumar, Bharat B Aggarwal
Feb 3, 2009·Stroke; a Journal of Cerebral Circulation·Brad R S BroughtonChristopher G Sobey
Dec 24, 2009·Journal of Neurochemistry·Tae Gen SonMark P Mattson
Apr 7, 2010·Archives of Biochemistry and Biophysics·Albena T Dinkova-Kostova, Paul Talalay
Apr 13, 2010·Experimental Neurology·Yoshihito SakataSylvain Doré
Apr 27, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Zoltan UngvariAnna Csiszar
Jul 27, 2010·Journal of Neuroimmunology·Jin A ShinEun-Mi Park
Aug 14, 2010·Current Drug Targets·William Durante
Jan 26, 2011·Annals of the New York Academy of Sciences·Goran PetrovskiDipak K Das
Feb 22, 2012·Immune Network·Hyo Jeong KimHun Taeg Chung
May 29, 2012·Archives of Biochemistry and Biophysics·A MovahedT Netticadan
Sep 10, 2014·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Ran WangLing Wang
Sep 29, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Fanren TangQin Yang

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Citations

Jan 16, 2020·Current Pharmaceutical Design·Joana BickerAmílcar Falcão
Jul 18, 2020·Oxidative Medicine and Cellular Longevity·Angélica Coyoy-SalgadoCarlos E Orozco-Barrios
May 11, 2021·Frontiers in Pharmacology·Qian XieDaoyin Gong
Jul 15, 2021·Oxidative Medicine and Cellular Longevity·Yuan ZhouXiang Fan
Aug 3, 2021·Progress in Neurobiology·Sebastiano Antonio RizzoBen Newland
Aug 8, 2021·International Journal of Molecular Sciences·Natalia CichonMichal Bijak
Aug 28, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcelo FarinaLuciano Saso

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Quantity One
SPSS

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