RET isoform-specific interaction with scaffold protein Ezrin promotes cell migration and chemotaxis in lung adenocarcinoma.

Lung Cancer : Journal of the International Association for the Study of Lung Cancer
Serisha MoodleyLois M Mulligan

Abstract

Increased expression of REarranged during Transfection (RET) kinase is reported in 10-20 % of lung adenocarcinomas (LUAD) and is associated with metastasis and reduced survival. Ezrin is a scaffold protein that promotes protein interactions with the actin cytoskeleton to regulate cell migration and is also associated with invasion and metastasis in cancers. RET isoforms interact with unique combinations of scaffold proteins to promote distinct signaling pathways. We hypothesized that RET isoforms associate distinctly with Ezrin for cytoskeletal reorganization and LUAD cell migration processes. HCC1833 and A549 LUAD, SH-SY5Y neuroblastoma or HEK-293 cells expressing RET and Ezrin were stimulated with the RET ligand glial cell line-derived neurotrophic factor (GDNF) and treated with RET, Ezrin or Src inhibitors. Co-immunoprecipitation or pull-down assays coupled to immunoblotting were used to investigate protein activation and interactions. Immunofluorescence confocal microscopy assessed LUAD cytoskeletal reorganization and colocalization of RET and Ezrin. Live-cell fluorescence imaging was used to measure cell migration and chemotaxis. GDNF promoted activation, interaction and colocalization of RET51 isoform and Ezrin. Inhibitio...Continue Reading

References

Feb 12, 1998·Science·T Pawson, J D Scott
Mar 22, 2001·Nature Reviews. Neuroscience·L Luo
Apr 27, 2001·Seminars in Cancer Biology·L S Price, J G Collard
Mar 12, 2002·The Journal of Biological Chemistry·Toshifumi FukudaMasahide Takahashi
May 4, 2002·Nature Reviews. Neuroscience·Matti S Airaksinen, Mart Saarma
Jul 2, 2002·The Journal of Biological Chemistry·Brian A Tsui-PierchalaEugene M Johnson
Aug 15, 2002·Biochemical and Biophysical Research Communications·Maria Grazia BorrelloMarco A Pierotti
Feb 10, 2004·The Journal of Biological Chemistry·Mario EncinasEugene M Johnson
Jul 23, 2011·Nature Reviews. Cancer·Evanthia T RoussosAntonia Patsialou
Dec 6, 2011·Clinical Lung Cancer·Xiao-Qin ZhangJian-Ying Zhou
Jan 19, 2012·British Journal of Pharmacology·Joost C M UitdehaagGuido J R Zaman
Feb 14, 2012·Nature Medicine·Takashi KohnoTatsuhiro Shibata
Jul 28, 2012·Human Mutation·Brandy D HyndmanLois M Mulligan
Jan 15, 2014·Journal of Cell Science·Jarama Clucas, Ferran Valderrama
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Feb 25, 2014·Nature Reviews. Cancer·Lois M Mulligan
Jul 18, 2014·Nature Protocols·Roman Gorelik, Alexis Gautreau
Jul 18, 2015·Current Opinion in Cell Biology·Guillaume JacquemetJohanna Ivaska
Dec 5, 2015·Frontiers in Pharmacology·Svenja HartmannSusanne Lutz
Mar 5, 2016·Neoplasia : an International Journal for Oncology Research·Yasemin Saygideğer-KontAykut Üren
Mar 29, 2016·Cancer Metastasis Reviews·Helmut H Popper
Mar 31, 2016·Cancer Science·Motonobu SaitoTakashi Kohno
Nov 23, 2016·Endocrine-related Cancer·Eric Y LianLois M Mulligan
Jan 7, 2017·Methods : a Companion to Methods in Enzymology·Daniel SageMichael Unser
Apr 17, 2017·Trends in Cell Biology·Robert J EddyJohn S Condeelis
May 24, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yingru LiYouyuan Wang
Aug 11, 2017·Journal of Cell Science·Brandy D HyndmanLois M Mulligan
Apr 10, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Peng LiuSanbao Hu

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Nov 27, 2020·Frontiers in Cell and Developmental Biology·Yanan SongWei Xia

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