Retargeting a maize β-glucosidase to the vacuole--evidence from intact plants that zeatin-O-glucoside is stored in the vacuole

Phytochemistry
Nagavalli S KiranBřetislav Brzobohatý

Abstract

Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize β-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucos...Continue Reading

References

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Jan 16, 2010·Journal of Integrative Plant Biology·Toru KudoHitoshi Sakakibara

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Citations

Dec 27, 2015·Plant Science : an International Journal of Experimental Plant Biology·James R Ketudat CairnsJong-Seong Jeon
Jan 3, 2018·Journal of Experimental Botany·Zuzana GelováJan Hejátko

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