Retention and multilineage expression of human hematopoietic stem cells in human-sheep chimeras

Stem Cells
E D ZanjaniA W Flake

Abstract

We have taken advantage of the permissive environment of the early gestational age fetus to engraft human hematopoietic stem cells (HSC) into preimmune fetal lambs. The resulting chimeras exhibit long-term multilineage engraftment of human cells in the bone marrow (BM) and peripheral blood (PB). Long-term multilineage reconstitution of second generation recipients by human cells isolated from chimeric sheep BM indicates that the engraftment in this model involved long-term repopulating human HSC. The model appears to be sensitive enough to detect relatively small numbers of transplanted HSC from pre- and postnatal human sources. Finally, transplantation of mature T lymphocyte-containing cells from a variety of sources results in lethal graft-versus-host disease (GVHD), analogous to clinical BM transplantation, suggesting that, at least in some respects, the model is biologically relevant. The human-sheep model is promising and may have important advantages over murine models for the in vivo study of normal and abnormal hematopoiesis and as a potential assay system for human HSC.

References

Jun 1, 1990·European Journal of Immunology·U Theopold, G Köhler
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S J SzilvassyC J Eaves
Jun 30, 1989·Cell·R H Schwartz
Jul 1, 1988·Science·G J SpangrudeI L Weissman
Dec 23, 1988·Science·R NamikawaJ M McCune
Mar 1, 1994·The Journal of Clinical Investigation·E D ZanjaniM Tavassoli
Oct 3, 1953·Nature·R E BILLINGHAMP B MEDAWAR

❮ Previous
Next ❯

Citations

Nov 15, 1997·World Journal of Surgery·J FungT Starzl
Aug 11, 2000·Transplantation Proceedings·Y LiY Yang
May 7, 1997·Transfusion Science·B Péault, P Charbord
Jan 30, 2007·Nature Reviews. Immunology·Leonard D ShultzDale L Greiner
Aug 16, 2002·British Journal of Haematology·Isabel BarãoJoão L Ascensão
Nov 10, 2001·Annual Review of Biomedical Engineering·L K Nielsen
Jan 1, 1997·Stem Cells·E D ZanjaniA W Flake
Jun 9, 1998·Stem Cells·D L GreinerL D Shultz
May 19, 2001·Blood Cells, Molecules & Diseases·S M OppenheimG B Anderson
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Robert E RichardC Anthony Blau
Aug 22, 2018·Human Vaccines & Immunotherapeutics·Feng HuangSong Guo Zheng
Oct 1, 1996·Trends in Biotechnology·T A McAdamsE T Papoutsakis

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.