Retention Behavior of Inorganic Anions in Hydrophilic Interaction Chromatography

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Nobuyuki TakayamaToyohide Takeuchi

Abstract

The retention behavior of inorganic anions was studied in hydrophilic interaction chromatography (HILIC). In this study, five kinds of HILIC stationary phases (amino, imidazole, amide, pyridine and zwitterionic) were investigated. It was found that only amino and imidazole columns exhibited the separation of inorganic anions under HILIC conditions. The retention mechanism was further investigated under both columns. A reversed elution order of inorganic anions was observed under the HILIC condition compared with those observed under the ion-exchange chromatography mode (IEC). The effect of salt species and their concentration in the eluent were investigated under constant acetonitrile (ACN) content. Sodium chloride and sodium perchlorate were chosen as the salt, and the salt (sodium perchlorate) concentration was varied from 10 to 40 mM to confirm the effect of the electrostatic interaction. The slope values of the plots of the log retention factor (k) versus the log eluent concentration were calculated to be between -0.43 and -0.45 for the amino column, while those obtained on the imidazole column were between -0.68 and -0.73. Various concentrations of ACN were also examined with 20 mM sodium perchlorate, and the typical HILIC...Continue Reading

References

Apr 23, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toyohide TakeuchiLee Wah Lim
Sep 14, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Toyohide Takeuchi, Lee Wah Lim
Jan 22, 2011·Journal of Chromatography. a·Lukas Sonnenschein, Andreas Seubert
Aug 31, 2011·Analytical and Bioanalytical Chemistry·Toyohide TakeuchiLee Wah Lim
Nov 13, 2012·Talanta·Mohammed E A Ibrahim, Charles A Lucy
Nov 28, 2012·Journal of Chromatography. a·Kaori AraiKazuhiko Tanaka
Dec 12, 2012·Physical Review Letters·M AblikimUNKNOWN BESIII Collaboration
Oct 7, 2014·The Analyst·S SandronB Paull

❮ Previous
Next ❯

Citations

Jun 25, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sakura YoshiiHideyuki Itabashi
Sep 13, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shinya Kitagawa
Apr 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hengye LiWei Wang
Nov 9, 2018·Analytical Chemistry·Valentina D'AtriDavy Guillarme

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Toyohide TakeuchiLee Wah Lim
Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Xiao-Lin JiangToyohide Takeuchi
© 2022 Meta ULC. All rights reserved