Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF

Biochemical and Biophysical Research Communications
S TsutsumiY Kato

Abstract

Mesenchymal stem cells (MSC) that can differentiate to various connective tissue cells may be useful for autologous cell transplantation to defects of bone, cartilage, and tendon, if MSC can be expanded in vitro. However, a short life span of MSC and a reduction in their differentiation potential in culture have limited their clinical application. The purpose of this study is to identify a growth factor(s) involved in self-renewal of MSC and the maintenance of their multilineage differentiation potential. Fibroblast growth factor-2 (FGF-2) markedly increased the growth rate and the life span of rabbit, canine, and human bone marrow MSC in monolayer cultures. This effect of FGF-2 was more prominent in low-density cultures than in high-density cultures. In addition, all MSC expanded in vitro with FGF-2, but not without FGF-2, differentiated to chondrocytes in pellet cultures. The FGF+ MSC also retained the osteogenic and adipogenic potential throughout many mitotic divisions. These findings suggest that FGFs play a crucial role in self-renewal of MSC.

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Citations

Oct 18, 2011·Cytotechnology·Hiroto MiwaMutsumi Takagi
Mar 14, 2007·Stem Cells and Development·Yoshie OzakiYukio Kato
Dec 6, 2008·Stem Cells and Development·Shigejiro IwashimaSeiichi Matsuo
May 24, 2007·Tissue Engineering·Arimichi OkazakiYasuhiko Tabata
Sep 2, 2008·Tissue Engineering. Part a·Wakako TsujiMasakazu Toi
Mar 20, 2010·Tissue Engineering. Part a·Jin Yu LiuStelios T Andreadis
Jun 14, 2008·BMC Musculoskeletal Disorders·Takeshi TeramuraChiaki Hamanishi
Oct 28, 2010·Stem Cell Research & Therapy·Melanie RodriguesAlan Wells
Jul 26, 2008·Development·Michael J GeskeThaddeus S Stappenbeck
Sep 26, 2006·Circulation Journal : Official Journal of the Japanese Circulation Society·Takashi UmemuraYukihito Higashi
Nov 2, 2004·Journal of Periodontology·Hiroyuki KawaguchiHidemi Kurihara
Jun 1, 2006·Journal of Periodontology·Naohiko HasegawaHidemi Kurihara

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