Retention of promutagenic O6-ethylguanine in the DNA of various rat tissues following transplacental inoculation with ethylnitrosourea

Cancer Letters
M J ChangA Koestner

Abstract

Pregnant Fischer rats at their 20th day of gestation were inoculated intravenously with [14C]ENU (75 mg/kg). The O6-ethylguanine content of liver, kidney, and brain DNA from both fetuses (offspring) and adults were quantitatively determined 3 h and 7 days after carcinogen treatment. Removal of O6-ethylguanine from the DNA of brain (target) was much slower than from liver (non-target) and kidney (low-target). Retention of O6-ethylguanine was found to be relatively similar in adult and fetal brains, although 100% of the offspring transplacentally exposed to 75 mg/kg ENU were shown to develop tumors of the nervous system, whereas the inoculated pregnant dams did not.

References

Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R Goth, M F Rajewsky

Citations

Jan 1, 1992·Teratogenesis, Carcinogenesis, and Mutagenesis·P H Huang, A Catalano
Sep 1, 1983·Chemico-biological Interactions·K Marushige, Y Marushige
Nov 13, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zoulikha M ZaïrGeorge E Johnson
Feb 1, 1982·Photochemistry and Photobiology·R E Gibson, S M D'Ambrosio
Sep 1, 1983·Environmental Health Perspectives·M J ChangR W Hart

Related Concepts

6-ethylguanine
Alkylation
Metazoa
Brain
DNA, Double-Stranded
DNA Replication
Ethylnitrosourea
Guanine
Kidney
Liver

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