PMID: 5919448Nov 11, 1966

Retention of the biological potency of human pituitary growth hormone after reduction and carbamidomethylation

Science
J S Dixon, C H Li

Abstract

The reduced alkylated derivative of human pituitary growth hormone was prepared by reacting the native hormone in 8M urea solution, first with mercaptoethanol and then with iodoacetamide. On the basis of amino acid analyses, the product isolated after gel filtration on Sephadex G-100 is completely devoid of unreduced cystine but otherwise has a composition identical to that of the native hormone. When assayed in both the rat tibia test and the pigeon crop-sac assay,it appears that the full potency of the native hormone is retained in the reduced alkylated derivative.

Citations

Jan 1, 1976·Pharmacology & Therapeutics. Part B: General & Systematic Pharmacology·J A SantomeA C Paladini
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C H Li, L Gráf
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·C R ReaganA E Wilhelmi
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C H Li, T A Bewley
Oct 1, 1967·Journal of Neurochemistry·D M Dawson
Aug 1, 1968·Journal of Neurochemistry·G R Dutton, H R Mahler
Feb 19, 1968·Biochimica Et Biophysica Acta·T A BewleyC H Li
May 15, 1971·International Journal of Cancer. Journal International Du Cancer·A ItoJ Furth
Oct 1, 1974·Archives of Biochemistry and Biophysics·W C ClarkeC H Li
Aug 1, 1969·Archives of Biochemistry and Biophysics·C H LiW K Liu
Sep 1, 1974·Metabolism: Clinical and Experimental·J L Kostyo
May 10, 1968·Biochemical and Biophysical Research Communications·W K Liu, J Meienhofer
Jan 30, 1977·Archives of Biochemistry and Biophysics·R A HoughtenC H Li
Dec 1, 1969·Biochemistry·J Brovetto-Cruz, C H Li
Feb 17, 2005·The Journal of Clinical Endocrinology and Metabolism·Amélie BessonPrimus E Mullis
Mar 6, 2020·The Journal of Biological Chemistry·Max SanderChristian J Strasburger

Related Concepts

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Iodoacetic Acids
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Recombinant Growth Hormone
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