PMID: 4905673Feb 1, 1970Paper

Retention or viral antigen in the cytoplasm of cells infected with temperature-sensitive mutants of an avian adenovirus

Proceedings of the National Academy of Sciences of the United States of America
M Ishibashi

Abstract

Immunofluorescent studies were made on chicken kidney cell cultures infected with various temperature-sensitive (ts) mutants of an avian adenovirus, chicken embryo lethal orphan (CELO), which grow well at the nonrestrictive temperature of 31 degrees , but fail to grow at the restrictive temperature of 40 degrees . At 40 degrees some mutants (ts 5, 8, and 10) accumulated viral antigen in the cytoplasm, but scarcely at all in the nucleus. However, at 31 degrees they accumulated the antigen in the nucleus like the wild-type strain at either 31 degrees or 40 degrees . These mutants seemed to complement each other in nuclear accumulation of antigen at 40 degrees . In cells infected with ts 8, the antigen remaining in the cytoplasm during incubation at 40 degrees was partially transferred to the nucleus even in the presence of an inhibitor of protein synthesis when the temperature was lowered to 31 degrees . The results suggest that viral antigen produced in the cytoplasm is transported to the nucleus and that these ts mutants are defective in this process at 40 degrees .There are apparently two other types of ts mutant: one type (ts 3, 7, and 12), like the wild-type strain, showing nuclear accumulation of antigen at 40 degrees and t...Continue Reading

References

Feb 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·J D LevinthalJ J Trentin
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·L F Velicer, H S Ginsberg
Mar 15, 1967·International Journal of Cancer. Journal International Du Cancer·J D LevinthalR Wicker
Aug 30, 1967·Journal of Ultrastructure Research·H F StichD S Yohn
Nov 1, 1963·Proceedings of the Society for Experimental Biology and Medicine·S K DUTTA, B S POMEROY
Oct 1, 1964·The Journal of Experimental Medicine·J H POPE, W P ROWE
Jun 17, 1964·Archiv für die gesamte Virusforschung·M PETEKG BERSANI
Jan 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·M D HOGGANR J HUEBNER

❮ Previous
Next ❯

Citations

Jan 1, 1973·Archiv für die gesamte Virusforschung·M J Guentzel, J S Butel
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·H HandaH Shimojo
Mar 1, 2019·Journal of Virology·James M Pipas
Sep 1, 1973·Journal of Virology·H S GinsbergH Shimojo
Aug 1, 1976·Journal of Virology·R S Kauffman, H S Ginsberg

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.