Retention pattern profiling of fungal metabolites on mixed-mode reversed-phase/weak anion exchange stationary phases in comparison to reversed-phase and weak anion exchange separation materials by liquid chromatography-electrospray ionisation-tandem mass spectrometry

Journal of Chromatography. a
Elisabeth ApfelthalerRainer Schuhmacher

Abstract

Retention properties of 79 fungal metabolites (including neutral, acidic, basic, and amphoteric compounds) were evaluated on distinct mixed-mode reversed-phase/weak anion exchange (RP/WAX)-type stationary phases by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) in gradient as well as in isocratic elution mode. The RP/WAX separation materials were prepared by functionalising thiol-modified silica with N-(10-undecenoyl)-3-aminoquinuclidine and N-(10-undecenoyl)-3-alpha-aminotropane, respectively. To evaluate complementarity in chromatographic selectivity the physico-chemically heterogeneous solute set was analysed also on a RP phase (C(18)), an amino-type WAX phase, and a commercially available RP/WAX-like mixed-mode phase. Analytes may interact with the RP/WAX ligands via (attractive/repulsive) ionic, RP-like hydrophobic, as well as hydrophilic (HILIC) retention mechanisms. Individual interactive increments were found to be basically controlled by the nature and amount of organic modifier, pH value of eluent, and ionic strength of buffer additives. It could be demonstrated that RP/WAX columns offer the potential to separate compounds by exploiting a combination of various chromatographic in...Continue Reading

References

Jul 5, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Wolfgang BickerWolfgang Lindner
Oct 4, 2005·Journal of the American Society for Mass Spectrometry·Eduard Rogatsky, Daniel Stein
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Mar 1, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·M De BoevreS De Saeger
Apr 1, 2009·International Journal of Molecular Sciences·Irena Kralj Cigić, Helena Prosen
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Aug 12, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Isao NadaokaToshimasa Toyo'oka
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May 17, 2021·Journal of Chromatography. a·Fernando Pradanas-GonzálezMaría Cruz Moreno-Bondi

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