Retinal analog study of the role of steric interactions in the excited state isomerization dynamics of rhodopsin

Biochemistry
G G KochendoerferRichard A Mathies

Abstract

The role of intramolecular steric interactions in the isomerization of the 11-cis-retinal chromophore in the photoreceptor protein rhodopsin is examined with resonance Raman and CD spectroscopy combined with quantum yield experiments. The resonance Raman spectra and CD spectra of 13-demethylrhodopsin indicate that its chromophore, an analog in which the nonbonded interaction between the 10-H and the 13-CH3 groups is removed, is less distorted in the C10...C13 region than the native chromophore. The reduced torsional and hydrogen-out-of-plane resonance Raman intensities further indicate that the excited state potential energy surface has a much shallower slope along the isomerization coordinate. This is consistent with the decrease in quantum yield from 0.67 in rhodopsin to 0.47 in 13-demethylrhodopsin. The resonance Raman intensities show that the steric twist is reintroduced by addition of a methyl group at the C10 position. However, the quantum yield of 10-methyl-13-demethylrhodopsin is found to be only 0.35. This is attributed to nonisomorphous protein-analog interactions. The nonbonded interaction between the 10-hydrogen and the 13-methyl group in 11-cis-retinal makes this isomer particularly effective as the light-sensing ...Continue Reading

References

Sep 4, 1974·Journal of the American Chemical Society·A Warshel, M Karplus
Feb 26, 1974·Biochemistry·R RowanM Karplus
Nov 20, 1953·The Journal of General Physiology·G WALD, P K BROWN

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Citations

Nov 14, 2001·Biochemistry·J E KimR A Mathies
Nov 10, 2011·Journal of the American Chemical Society·Amir WandSanford Ruhman
Oct 27, 2011·Journal of the American Chemical Society·Sivakumar Sekharan, Keiji Morokuma
Dec 10, 2009·Journal of the American Chemical Society·Petra H M Bovee-GeurtsWillem J Degrip
Apr 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrey V StrutsMichael F Brown
Jun 11, 2011·The Journal of Biological Chemistry·Jian LiuElsa C Y Yan
Mar 3, 2010·Annual Review of Biophysics·Steven O Smith
Apr 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alessandro CembranMarco Garavelli
Jan 22, 2013·Annual Review of Physical Chemistry·Amir WandSanford Ruhman
Feb 11, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Andreas OckenfelsWolfgang Gärtner
Dec 25, 2013·Chemical Reviews·Oliver P ErnstHideki Kandori
May 11, 2005·Protein Science : a Publication of the Protein Society·Anatoly P DubnovitskyAnastassios C Papageorgiou
Apr 10, 2004·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Nathan FishkinKoji Nakanishi
Oct 20, 2004·Journal of Molecular Biology·Jade LiGebhard F X Schertler
Sep 17, 2015·Journal of the American Chemical Society·Giovanni BassolinoPhilipp Kukura
Sep 1, 2009·Biochimica Et Biophysica Acta·Michael F BrownAndrey V Struts
Nov 5, 2013·Biochimica Et Biophysica Acta·Andreyah PopeSteven O Smith
Jun 16, 2015·Physical Chemistry Chemical Physics : PCCP·Elżbieta Walczak, Tadeusz Andruniów
Mar 24, 2015·Journal of Magnetic Resonance·Naoki KimataSteven O Smith
Nov 21, 2007·Biochimica Et Biophysica Acta·Michael F BrownIngrid Wallat
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Thomas P SakmarElias S Awad
Apr 16, 2016·The Journal of Physical Chemistry. a·Igor Schapiro
Feb 18, 2017·Physical Review. E·L MusbatM Sheves
Oct 3, 2001·Physiological Reviews·S T MenonT P Sakmar
Nov 30, 2018·Physical Chemistry Chemical Physics : PCCP·Partha Pratim RoyTiago Buckup
Nov 12, 2020·Nature Communications·Matthias BroserPeter Hegemann
Mar 29, 2007·The Journal of Physical Chemistry. B·Oliver WeingartVolker Buss
Feb 1, 2007·Journal of the American Chemical Society·Sivakumar SekharanVolker Buss
Nov 26, 2004·Journal of the American Chemical Society·Ute F RöhrigUrsula Rothlisberger

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