Retinal and Cortical Blood Flow Dynamics Following Systemic Blood-Neural Barrier Disruption

Frontiers in Neuroscience
Flora HuiBang V Bui

Abstract

To consider whether imaging retinal vasculature may be used as a marker for cortical vessels, we compared fluorescein angiography flow dynamics before and after pharmacological disruption of blood-neural barriers. Sodium fluorescein (1%, 200 μl/kg) was intravenously delivered in anesthetized adult Long Evans rats (n = 44, brain = 18, retina = 26). In the brain cohort, a cranial window was created to allow direct visualization of surface cortical vessels. Video fluorescein angiography was captured using a rodent retinal camera at 30 frames/second and fluorescence intensity profiles were evaluated for the time to reach 50% brightness (half-rise), 50% decay (half-fall), and the plateau level of remnant fluorescence (offset, %). Cortical vessels fluoresced earlier (artery half-rise: 5.6 ± 0.2 s) and decayed faster (half-fall: 10.3 ± 0.2 s) compared to retinal vasculature. Cortical vessels also had a considerably higher offset, particularly in the capillaries/extravascular space (41.4 ± 2.7%) whereas pigment in the retina reduces such residual fluorescence. In a sub-cohort of animals, sodium deoxycholate (DOC, 0.06 M dissolved in sterile saline, 1 mL) was delivered intravenously to cause simultaneous disruption of the blood-brain an...Continue Reading

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Citations

Jun 30, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Junyi ShenHui Cai
Jun 28, 2019·Stroke; a Journal of Cerebral Circulation·Hung NguyenCesar V Borlongan
Oct 13, 2019·Experimental Biology and Medicine·Matt HeyckCesar V Borlongan
Jul 8, 2020·Pharmaceutics·Jeffrey FarooqCesar V Borlongan
Dec 12, 2019·Neural Regeneration Research·Chase KingsburyCesar V Borlongan
Aug 25, 2020·Translational Vision Science & Technology·Rachel S ChongLeopold Schmetterer
Aug 31, 2020·The Journal of Pharmacology and Experimental Therapeutics·Kelsey NorthAlex Dopico
Feb 23, 2021·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Jorge G ArroyoColin A Lemire

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