Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro

Experimental Cell Research
Isai MathivananVolker Enzmann

Abstract

The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by magnetic-activated cell sorting (MACS) for specific stem cell subsets (CD34(+)CD38(+)/CD34(+)CD38(-)). These cells were then co-cultured on human retinal pigment epithelial cells (hRPE) for 7 days. The expression of stem cell, neural and retina-specific markers was examined by immunostaining, and the gene expression profiles were assessed after FACS separation of the co-cultured hBMSCs by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Furthermore, in vitro functionality of the differentiated cells was analyzed by quantifying phagocytosis of CY5-labeled photoreceptor outer segments (POS). After 7 days of co-culture, hBMSCs adopted an elongated epithelial-like morphology and expressed RPE-specific markers, such as RPE65 and bestrophin. In addition, these differentiated cells were able to phagocytose OS, one of the main characteristics of native RPE cells. Our data demonstrated that human CD34(+)...Continue Reading

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Citations

Oct 6, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Vianney DelplaceMolly Shoichet
Aug 7, 2016·Journal of Tissue Engineering·Aaron H Fronk, Elizabeth Vargis
Nov 9, 2017·Cell Transplantation·Vladimir HolanJan Kossl
Jun 18, 2019·Tissue Engineering and Regenerative Medicine·Hamid Aboutaleb KadkhodaeianTaher Taheri
Apr 14, 2021·Journal of Cellular Biochemistry·Hoda Shams NajafabadiEhsan Ranaei Pirmardan

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