Retinal ganglion cell line apoptosis induced by hydrostatic pressure

Brain Research
Ashish AgarMark A Hill

Abstract

Cellular responses to changes in pressure are implicated in numerous disease processes. In glaucoma apoptosis of retinal ganglion cells (RGCs) is associated with elevated intra-ocular pressure, however, the exact cellular mechanisms remain unclear. We have previously shown that pressure can induce apoptosis in B35 and PC12 neuronal cell lines, using an in vitro model for pressure elevation. A novel RGC line allows us to study the effects of pressure on retinal neurons. 'RGC-5' cultures were subjected to elevated ambient hydrostatic pressure conditions in our model. Experimental pressure conditions were 100 mm Hg and 30 mm Hg, representing acute (high) and chronic (lower-pressure) glaucoma, and 15 mm Hg for normal intra-ocular pressure, set above atmospheric pressure for 2 h. Negative controls were treated identically except for the application of pressure, while positive controls were generated by treatment with a known apoptotic stimulus. Apoptosis was determined by a combination of cell morphology and specific TUNEL and Annexin V fluorescent markers. These were assessed simultaneously by laser scanning cytometry (LSC), which also enabled quantitative marker analysis. RGC-5 neurons showed a significantly increased proportion o...Continue Reading

References

Oct 11, 1992·Trends in Pharmacological Sciences·O P HamillD W McBride
Nov 1, 1991·Experimental Eye Research·N TakahashiJ Caprioli
Dec 1, 1973·The Journal of Pathology·A H WyllieA R Currie
Sep 1, 1983·Clinical Orthopaedics and Related Research·R H GelbermanM A Minteer-Convery
Oct 1, 1982·Archives of Ophthalmology·R L Radius, B Bade
Jul 1, 1993·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C L HaskinI L Cameron
Jul 1, 1995·Experimental Eye Research·E Garcia-ValenzuelaS C Sharma
Jul 1, 1993·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R GoldH Lassmann
Aug 25, 1997·Experimental Cell Research·K J TakanoT Satou
Mar 12, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·H D MennelJ Wenzel
Jan 9, 1999·The Journal of Biological Chemistry·F MaingretE Honoré
Jan 27, 1999·Progress in Retinal and Eye Research·H A Quigley
Jul 22, 1999·The British Journal of Ophthalmology·N N OsborneM S Nash
Mar 24, 2000·The British Journal of Ophthalmology·M B WaxM R Hernandez
May 8, 2000·Journal of Neuroscience Research·A AgarM T Coroneo
Feb 13, 2001·Brain Research. Molecular Brain Research·R R KrishnamoorthyN Agarwal
Jun 19, 2001·The Japanese Journal of Physiology·J TakeS Terada
Jun 26, 2003·Investigative Ophthalmology & Visual Science·Paul AounNeeraj Agarwal
Nov 26, 2003·Investigative Ophthalmology & Visual Science·Zachary S BoydRajkumar V Patil
Apr 6, 2005·Clinical & Experimental Ophthalmology·Freny B KalapesiMinas T Coroneo
Jun 1, 2005·The British Journal of Ophthalmology·F B KalapesiM A Hill
Feb 21, 2006·The British Journal of Ophthalmology·J C H TanM T Coroneo
Apr 28, 2006·Brain Research·Ashish AgarMark A Hill

❮ Previous
Next ❯

Citations

Nov 10, 2010·Cellular and Molecular Neurobiology·Miryam A FragosoAbigail S Hackam
Feb 13, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Fabrizio GiansantiUgo Menchini
May 13, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Daniel A RyskampDavid Krizaj
Jan 1, 2011·Medical Devices : Evidence and Research·Katherine VorvolakosSteven K Pollack
Feb 11, 2014·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Fabrizio GiansantiUgo Menchini
Oct 23, 2013·Current Eye Research·David KrižajValery I Shestopalov
Feb 5, 2008·Experimental Eye Research·Nathan Rock, Shravan K Chintala
Jul 27, 2007·Clinical & Experimental Ophthalmology·Brian T Kent-Smith
Jan 25, 2011·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Zheng HeBang V Bui
Apr 20, 2016·European Journal of Pharmacology·Nadine Von Thun Und Hohenstein-BlaulFranz H Grus
Jun 13, 2015·BioMed Research International·Makoto IshikawaYukitoshi Izumi
Apr 2, 2013·Progress in Retinal and Eye Research·Katharina BellFranz H Grus
Apr 30, 2016·The American Journal of Medicine·Antonio GrecoMarco de Vincentiis
Aug 1, 2016·Immunologic Research·Maria Ida RizzoMarco de Vincentiis
Apr 28, 2006·Brain Research·Ashish AgarMark A Hill
Oct 11, 2016·Current Eye Research·Katharina BellFranz H Grus
Apr 18, 2007·Journal of Glaucoma·Domalapalli Maneesh Kumar, Neeraj Agarwal
Dec 1, 2017·International Ophthalmology·Alexandre de Carvalho Mendes Paiva, Adenilson de Souza da Fonseca
Aug 18, 2020·Brain and Behavior·Fatemeh NafianNarsis Daftarian
Jan 1, 2008·Biomarker Insights·Anja-Kristina BrustFranz H Grus
May 16, 2019·Tissue Engineering. Part B, Reviews·Pascal A M M VroemenJan de Boer
Nov 9, 2019·Applied Mechanics Reviews·Shaobao LiuFeng Xu
May 30, 2020·Micromachines·Yoonhee HaSang Beom Jun
May 16, 2019·Experimental and Therapeutic Medicine·Peng RenXiao-Fei Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved