Retinal ganglion cell population in adult albino and pigmented mice: a computerized analysis of the entire population and its spatial distribution

Vision Research
M Salinas-NavarroM Vidal-Sanz

Abstract

In adult Swiss albino and C57 pigmented mice, RGCs were identified with a retrogradely transported neuronal tracer applied to both optic nerves (ON) or superior colliculi (SCi). After histological processing, the retinas were prepared as whole-mounts, examined and photographed under a fluorescence microscope equipped with a motorized stage controlled by a commercial computer image analysis system: Image-Pro Plus((R)) (IPP). Retinas were imaged as a stack of 24-bit color images (140 frames per retina) using IPP with the Scope-Pro plug-in 5.0 and the images montaged to create a high-resolution composite of the retinal whole-mount when required. Single images were also processed by specific macros written in IPP that apply a sequence of filters and transformations in order to separate individual cells for automatic counting. Cell counts were later transferred to a spreadsheet for statistical analysis and used to generate a RGC density map for each retina. The mean total numbers of RGCs labeled from the ON, in Swiss (49,493+/-3936; n=18) or C57 mice (42,658+/-1540; n=10) were slightly higher than the mean numbers of RGCs labeled from the SCi, in Swiss (48,733+/-3954; n=43) or C57 mice (41,192+/-2821; n=42), respectively. RGCs were ...Continue Reading

References

Jan 1, 1979·Annual Review of Neuroscience·R W Rodieck
Jun 1, 1990·Visual Neuroscience·G W Balkema, U C Dräger
Jan 1, 1989·Visual Neuroscience·L C SilveiraE Oswaldo-Cruz
Jan 1, 1986·Experimental Brain Research·B E Reese, A Cowey
Feb 1, 1969·The Journal of Comparative Neurology·R D Lund
Aug 1, 1983·Brain Research·R Linden, V H Perry
Sep 20, 1983·The Journal of Comparative Neurology·V H PerryR Linden
Feb 1, 1982·Experimental Neurology·Y FukudaT Shirokawa
Feb 15, 1980·The Journal of Comparative Neurology·R D LundJ Boles
Jun 1, 1980·The Journal of Comparative Neurology·U C Dräger, J F Olsen
Aug 12, 1996·Neuroreport·M P Villegas-PérezR D Lund
May 30, 2001·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S L SchmidtR Linden
Nov 14, 2002·Behavioural Brain Research·Glen T PruskyIan Q Whishaw
May 27, 2004·Investigative Ophthalmology & Visual Science·Fumihiko MabuchiRobert N Weinreb
Feb 25, 2005·Investigative Ophthalmology & Visual Science·May-Britt TessemAnna Midelfart
Sep 28, 2005·Investigative Ophthalmology & Visual Science·Sergio Mayor-TorroglosaManuel Vidal-Sanz
Oct 26, 2005·The Journal of Cell Biology·Tatjana C JakobsRichard H Masland
Dec 16, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lely A QuinaEric E Turner
Dec 31, 2005·Investigative Ophthalmology & Visual Science·Melissa M HamiltonLisa L Wei
Jun 22, 2006·Neurotoxicity Research·M Vidal-SanzM P Villegas-Perez
Jul 21, 2006·The Journal of Comparative Neurology·Marco Antonio Marco-GomarizMaría Paz Villegas-Pérez
Sep 28, 2006·Investigative Ophthalmology & Visual Science·Hiroshi SakaiBeatrice Y J T Yue
Jan 2, 2007·Investigative Ophthalmology & Visual Science·Maureen G Maguire, UNKNOWN Vision in Preschoolers Study Group
Apr 27, 2007·Investigative Ophthalmology & Visual Science·Steven L BernsteinShalom E Kelman
Aug 25, 2007·Molecular and Cellular Neurosciences·J A MurphyD B Clarke
Dec 26, 2007·The Journal of Cell Biology·Gareth R HowellSimon W M John
Jan 4, 2008·Investigative Ophthalmology & Visual Science·Kelly K PertilePaul N Baird
Jan 11, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ileana SotoNicholas Marsh-Armstrong
Feb 5, 2008·Journal of Neuroscience Methods·John B FiletaCynthia L Grosskreutz

❮ Previous
Next ❯

Citations

Dec 3, 2014·Progress in Retinal and Eye Research·Corey A Smith, Balwantray C Chauhan
Nov 4, 2011·Journal of Neurophysiology·Mark H HistedJohn H R Maunsell
Jan 24, 2015·Current Eye Research·Eline DekeysterLieve Moons
Jul 1, 2015·Current Eye Research·Eamonn T FahyJonathan G Crowston
Feb 15, 2011·Experimental Eye Research·M C Sánchez-MigallónM Agudo-Barriuso
Oct 20, 2010·Experimental Eye Research·Diego García-AyusoMaría P Villegas-Pérez
Aug 19, 2015·Experimental Eye Research·Adam Hedberg-BuenzMichael G Anderson
Jun 14, 2015·Experimental Eye Research·Andrea C NuschkeBalwantray C Chauhan
Jun 29, 2012·The European Journal of Neuroscience·Naira YeritsyanSiegrid Löwel
Mar 19, 2014·The Journal of Comparative Neurology·Larissa MckettonKeith A Schneider
Dec 10, 2013·The Journal of Comparative Neurology·Allen R RodriguezNicholas C Brecha
Aug 22, 2015·Eye·S I BalendraM F Cordeiro
Nov 26, 2015·Applied Microbiology and Biotechnology·Songya LiLingyun Cao
Dec 11, 2013·Veterinary Ophthalmology·Hsiao-Hui WangJuliet R Gionfriddo
Nov 5, 2014·Developmental Neurobiology·J J Johannes HjorthStephen J Eglen
Oct 9, 2012·Current Opinion in Pharmacology·M Agudo-BarriusoM Vidal-Sanz
Mar 24, 2015·Experimental Eye Research·F M Nadal-NicolásM Agudo-Barriuso
Oct 18, 2015·Experimental Eye Research·Adam Hedberg-BuenzMichael G Anderson
Mar 1, 2011·Experimental Eye Research·C Galindo-RomeroM Agudo-Barriuso
Oct 24, 2014·Frontiers in Neuroanatomy·Francisco M Nadal-NicolásMarta Agudo-Barriuso
Mar 27, 2015·PloS One·Francisco J Valiente-SorianoManuel Vidal-Sanz
Aug 16, 2016·Experimental Eye Research·Ben Mead, Stanislav Tomarev
Aug 22, 2016·Acta Neuropathologica·Benjamin Michael DavisMaria Francesca Cordeiro
Mar 19, 2014·Experimental Eye Research·María J Pérez de LaraJesús Pintor
Dec 13, 2016·PloS One·Patrick ArbogastLeo Peichl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Polyendocrine Syndromes

This feed focuses on a rare genetic condition called Autoimmune Polyendocrine Syndromes, which are characterized by autoantibodies against multiple endocrine organs. This can lead to Type I Diabetes.

Autoimmune Polyendocrinopathies

Autoimmune polyendocrinopathies, also called polyglandular autoimmune syndromes (PGASs), or polyendocrine autoimmune syndromes(PASs), are a heterogeneous group of rare diseases characterized by autoimmune activity against more than one endocrine organ, although non-endocrine organs can be affected. Discover the latest research on autoimmune polyendocrinopathies here.