Retinal pigment epithelial cells promote spatial reorganization and differentiation of retina photoreceptors.

Journal of Neuroscience Research
Olga L GermanLuis E Politi

Abstract

Retina differentiation involves the acquisition of a precise layered arrangement, with RPE cells in the first layer in intimate contact with photoreceptors in the second layer. Here, we developed an in vitro coculture model, to test the hypothesis that RPE cells play a pivotal role in organizing the spatial structure of the retina. We cocultured rat retinal neurons with ARPE-19 epithelial cells under various experimental conditions. Strikingly, when seeded over RPE cells, photoreceptors attached to their apical surfaces and proceeded with their development, including the increased synthesis of rhodopsin. Conversely, when we seeded RPE cells over neurons, the RPE cells rapidly detached photoreceptors from their substrata and positioned themselves underneath, thus restoring the normal in vivo arrangement. Treatment with the metalloproteinase inhibitor TIMP-1 blocked this reorganization, suggesting the involvement of metalloproteinases in this process. Reorganization was highly selective for photoreceptors because 98% of photoreceptors but very few amacrine neurons were found to redistribute on top of RPE cells. Interestingly, RPE cells were much more efficient than other epithelial or nonepithelial cells in promoting this reorgan...Continue Reading

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Citations

Dec 1, 2010·Journal of Ocular Biology, Diseases, and Informatics·Kamla DuttYang Cao
Mar 23, 2013·Biology Open·Katy SchmidtDavid J Stephens
Nov 1, 2018·Clinical & Experimental Ophthalmology·Aparna MuraliChristopher J Layton
Nov 9, 2018·Frontiers in Physiology·Qian ZhangXiaojing Wang
Aug 28, 2020·International Journal of Molecular Sciences·Abdullah Al-AniMark Ungrin
Dec 28, 2019·Molecular Therapy : the Journal of the American Society of Gene Therapy·Min ZhengZongchao Han
Nov 3, 2020·Frontiers in Cellular Neuroscience·Ratnesh K Singh, Igor O Nasonkin
May 8, 2021·Frontiers in Neuroscience·Meimanat FathiZohreh Hosseinzadeh
Jun 5, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christin Hanke-GogokhiaEnrique Rodriguez-Boulan

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