Retinal removal up-regulates cannabinoid CB(1) receptors in the chick optic tectum

Journal of Neuroscience Research
Gabriela P ChavesAndréa S Torrão

Abstract

The endocannabinoid system has been implicated in several neurobiological processes, including neurodegeneration and neuroprotection. The aim of this study was to evaluate the effects of unilateral retinal ablation on the expression of the cannabinoid receptor subtype 1 (CB(1)) at both protein and mRNA levels in the optic tectum of the adult chick brain. After different survival times postlesion (2-30 days), the chick brains were subjected to immunohistochemical, immunoblotting, and real-time PCR procedures to evaluate CB(1) expression. TUNEL and Fluoro-Jade B were used to verify the possible occurrence of cell death, and immunostaining for the microtubule-associated protein MAP-2 was performed to verify possible dendritic remodeling after lesions. No cell death could be observed in the deafferented tectum, at least up to 30 days postlesion, although Fluoro-Jade B could reveal degenerating axons and terminals. Retinal ablation seems to generate an increase of CB(1) protein in the optic tectum and other retinorecipient visual areas, which paralleled an increase in MAP-2 staining. On the other hand, CB(1) mRNA levels were not changed after retinal ablation. Our results reveal that CB(1) expression in visual structures of the adul...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Jun 15, 1999·Progress in Neurobiology·A Ameri
Dec 14, 1999·The Journal of Comparative Neurology·S YazullaD G Deutsch
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·A StraikerG Maguire
Jan 13, 2000·Trends in Neurosciences·J Fernández-RuizJ A Ramos
Feb 13, 2001·Progress in Neurobiology·R G Pertwee
Oct 2, 2001·Journal of Neurochemistry·H H HansenH S Hansen
Feb 8, 2002·Molecular Neurobiology·R I GrundyM Beltramo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 22, 2002·Journal of Neurochemistry·Giovanni MarsicanoChristian Behl
May 31, 2002·Pharmacological Reviews·A C HowlettR G Pertwee
Jun 8, 2002·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E Fride
Nov 14, 2002·Molecular Neurobiology·Mario van der SteltJohannes F G Vliegenthart
Jun 28, 2003·Neurotoxicity Research·José A RamosJavier Fernández-Ruiz
Jul 5, 2003·Physiological Reviews·Tamas F FreundDaniele Piomelli
Oct 8, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T G Ramsay, R W Rosebrough
Nov 5, 2003·Nature Reviews. Neuroscience·Daniele Piomelli
Apr 14, 2004·The European Journal of Neuroscience·Leonid G KhaspekovBeat Lutz
Sep 17, 2004·Journal of Neuroscience Research·Andréa S Torrão, Luiz R G Britto
Oct 7, 2004·European Journal of Pharmacology·Ester Fride
Oct 7, 2004·Neuropharmacology·Allyn C HowlettLinda J Porrino
Feb 1, 2005·European Journal of Pharmacology·David A KaranianBen A Bahr
Feb 25, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David PanikashviliEsther Shohami
Apr 28, 2005·Nature Neuroscience·Vincenzo Di Marzo, Isabel Matias
Aug 16, 2005·Brain Research. Developmental Brain Research·Mauro LeonelliAndréa S Torrão
Jun 17, 2006·Annual Review of Neuroscience·Vivien ChevaleyrePablo E Castillo
Dec 5, 2006·Brain Research·Glenna L GilbertStanley A Thayer
Apr 4, 2007·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Yuki HashimotodaniMasanobu Kano

❮ Previous
Next ❯

Citations

Feb 18, 2016·Neural Plasticity·Thomas SchwitzerVincent Laprevote
Sep 12, 2016·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·John C BramleyWilliam J Buchser
Feb 17, 2009·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M LeonelliL R G Britto

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A StraikerG Maguire
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Ken SoderstromVincenzo Di Marzo
The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Mario Alexander Penzo, José Luis Peña
© 2021 Meta ULC. All rights reserved