Retinal toxicity of intravitreal genistein in a rabbit model

Retina
Tito FioreCarlo Cagini

Abstract

The purpose of this study was to evaluate the preclinical safety of intravitreal genistein in rabbit eyes over a short-term period. Twelve New Zealand albino rabbits were selected for this study. Four concentrations of genistein (LC Laboratories, Woburn, MA) were prepared: 24 mg/0.1 mL, 135 mg/0.1 mL, 270 mg/0.1 mL, and 540 mg/0.1 mL. Each concentration was injected intravitreally in one eye of three rabbits. As a control, the vehicle solution was injected into the other eye of each animal. Retinal safety of intravitreal genistein was studied with electroretinography and histologic examination in rabbits. Electroretinography recordings were made before the injection and 3 weeks after the injection. Eventually, the rabbits were killed and the retinas were examined by light microscopy. Immunohistochemical staining with caspase-3 and caspase-9 was also performed to evaluate apoptotic expression in all study and control eyes. Electroretinography studies showed no significant difference between control and genistein-injected eyes at any of the doses in the rabbit model. Histologic examination showed no retinal abnormality in the rabbits injected with different concentrations of genistein. Immunohistochemical staining with caspase-3 ...Continue Reading

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Citations

May 17, 2012·Chemical Research in Toxicology·Lu YanYong Liu
Apr 20, 2013·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Marilita M MoschosEmmanouil Agapitos
Mar 10, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Mimi LinLiming Dai

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