Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.

Biology of Reproduction
Elizabeth SnyderMichael D Griswold

Abstract

Throughout the reproductive lifespan of most male mammals, sperm production is constant because of the regulated differentiation of spermatogonia. Retinoic acid (RA) and a downstream target, Stra8, are required for complete spermatogenesis. To examine the role of RA in initiating spermatogonial differentiation, a transgenic mouse model expressing beta-galactosidase under the control of an RA response element was used. Cells in the neonatal testis undergoing active RA signaling were visualized by beta-galactosidase activity, the relationship between RA and differentiation determined, and the role of RA-degrading enzymes in regulating RA demonstrated. Beta-galactosidase activity was found to be predominantly associated with differentiating, premeiotic germ cells and to be distributed nonuniformly throughout the seminiferous tubules. Additionally, beta-galactosidase activity in premeiotic germ cells colocalized with STRA8 protein and was induced in germ cells with exogenous RA treatment. The RA-degrading enzyme, CYP26B1, was found to have germ cell localization and nonuniform distribution between tubules via immunohistochemistry. Treatment with a CYP26 enzyme inhibitor resulted in an increased number of germ cells with both beta-g...Continue Reading

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Citations

Jan 7, 2011·Biology of Reproduction·Cathryn A HogarthMichael D Griswold
Jan 14, 2011·Biology of Reproduction·Elizabeth M SnyderMichael D Griswold
Nov 15, 2011·Biology of Reproduction·Michael D GriswoldPeter Koopman
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Methods Mentioned

BETA
transgenic

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JMP

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