PMID: 8456086Jan 1, 1993Paper

Retinoic acid decreases attachment of JAR choriocarcinoma spheroids to a human endometrial cell monolayer in vitro

Placenta
N J JohnH W Denker

Abstract

The ability of retinoic acid (RA) to modulate attachment of JAR choriocarcinoma multicellular spheroids to monolayers of a human uterine epithelial cell line (RL95-2) was examined using a centrifugal force-based adhesion assay. Exposure of choriocarcinoma spheroids to RA (10(-7) to 10(-5) M) over a 3-day culture period resulted in a dose-dependent decrease of attachment. Significant decreases in attachment were detected after 30 min (75 per cent versus 25 per cent) and 1 h (92 per cent versus 26 per cent) of confrontation-culture between choriocarcinoma and uterine cells for control versus 10(-5) M RA; by 5 h 100 per cent spheroid attachment was detected in all treatment groups. RA had no effect on cell proliferation in JAR spheroids, but 10(-5) M RA treatment induced a fivefold increase in secretion of human chorionic gonadotrophin (hCG), a known marker of conversion of cytotrophoblast to syncytiotrophoblast-like cells. These findings demonstrate that RA modulates cellular attachment and differentiation in choriocarcinoma spheroids in vitro.

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Citations

Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·N J JohnH W Denker
Apr 1, 1997·The Journal of Obstetrics and Gynaecology Research·S YamadaY Tomoda
Feb 18, 2009·Reproductive Biology and Endocrinology : RB&E·Haggar HardufEliezer Shalev
Dec 26, 2009·Reproductive Biology and Endocrinology : RB&E·Karin TammMadis Metsis
Jun 1, 1993·In Vitro Cellular & Developmental Biology. Animal·N J JohnH W Denker

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