Retinoic acid enhances adhesiveness, laminin and integrin beta 1 synthesis, and retinoic acid receptor expression in F9 teratocarcinoma cells

Journal of Cellular Physiology
S A RossL M De Luca

Abstract

The teratocarcinoma-derived F9 cells respond to retinoic acid (RA) and RA plus dibutyrylcyclic adenosine monophosphate (dcAMP) by differentiating into endoderm cells, which elaborate a laminin and type IV collagen-rich matrix. We found that the induction of differentiation is accompanied by a small but consistent increase in cell adhesiveness to a variety of substrates, including laminin. Therefore we investigated biochemical mechanisms involved in this phenomenon. Endoglycosidase treatment showed that laminin contains complex and hybrid oligosaccharide structures. RA enhanced general biosynthesis of laminin without a specific increase in galactose incorporation: this sugar was mainly in polylactosamine structures in the A chain of laminin and as terminal galactose alpha 1,3 galactose in the B chain. Laminin receptor analysis showed that RA decreased laminin binding protein-37 (LBP-37) but increased the amount of beta 1 integrin, suggesting the involvement of beta 1 integrin in the attachment process. Northern blot analysis showed increased expression of retinoid receptors within hours of RA exposure. These studies demonstrate that RA increases cell to substrate interactions by increasing the biosynthesis of laminin and beta 1 ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 15, 1979·Experimental Cell Research·A M JettenJ P Poon
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C A MartinJ L Napoli
Oct 1, 1989·The Journal of Investigative Dermatology·R StadlerC E Orfanos
Dec 4, 1987·Journal of Immunological Methods·E N Dotsika, C J Sanderson
Jan 1, 1987·Annual Review of Cell Biology·G R Martin, R Timpl
Dec 1, 1987·Experimental Cell Research·S Kato, L M De Luca
Aug 6, 1986·Biochimica Et Biophysica Acta·R G ArumughamR A Laine
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Y Wang, L J Gudas
Nov 1, 1983·Archives of Biochemistry and Biophysics·C N RaoL A Liotta
Oct 15, 1980·Biochimica Et Biophysica Acta·K OldenK M Yamada
Apr 1, 1980·Environmental Health Perspectives·L M De LucaN Maestri

❮ Previous
Next ❯

Citations

Dec 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C A BurdsalD R McClay
May 3, 2014·Animal Reproduction Science·Qing-Qing ShiBi-Chun Li
Dec 1, 1994·Current Opinion in Cell Biology·J M Love, L J Gudas
Jan 30, 2015·Biological Reviews of the Cambridge Philosophical Society·Vincent DiGiacomo, Daniel Meruelo
Apr 20, 2000·JPEN. Journal of Parenteral and Enteral Nutrition·D A Swartz-BasileM S Levin
Sep 11, 2004·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Jörg Mey, Peter McCaffery
Oct 31, 2000·Microscopy Research and Technique·D AberdamP Simon-Assmann
Sep 1, 1996·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B P HierckR E Poelmann
Mar 6, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Laurent L OzbunSonia B Jakowlew
Jul 19, 2000·American Journal of Physiology. Heart and Circulatory Physiology·M M Medhora

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.