Retinoic acid-induced expression of apolipoprotein D and concomitant growth arrest in human breast cancer cells are mediated through a retinoic acid receptor RARalpha-dependent signaling pathway.

The Journal of Biological Chemistry
Y S López-BoadoC López-Otin

Abstract

Apolipoprotein D (apoD) is a human plasma protein, belonging to the lipocalin superfamily, that is produced by a specific subtype of highly differentiated breast carcinomas and that is strongly up-regulated by retinoic acid (RA) in breast cancer cells. In this work, we have examined the molecular mechanisms mediating the induction of apoD gene expression by retinoids in T-47D human breast cancer cells. Northern blot analysis revealed that Ro40-6055, a synthetic retinoid that selectively binds and activates the retinoic acid receptor RARalpha, induced the accumulation of apoD mRNA in breast cancer cells in a time- and dose-dependent manner. The time course analysis demonstrated that apoD mRNA was induced 14-fold over control cells after 48 h of incubation with 10(-8) M Ro40-6055. As little as 10(-11) M of this retinoid induced apoD mRNA 5-fold over the control, whereas incubation with 10(-7) M Ro40-6055 induced maximally 15-fold over control cells. RARalpha-selective antagonists counteracted the inductive effects of all-trans-RA, 9-cis-RA, and Ro40-6055 on the expression of apoD, when present at the same concentration as the retinoid agonists. By contrast, RARbeta-, RARgamma-, and RXR-selective retinoids did not affect apoD gene...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C ApfelM Klaus
Dec 18, 1992·Science·J M LehmannM Pfahl
Aug 1, 1991·Molecular and Cellular Biology·M HusmannM Pfahl
Jan 1, 1991·Progress in Lipid Research·P K WeechE Rassart
Dec 1, 1990·The Biochemical Journal·M CrettazW Hunziker
Jan 1, 1990·Annals of the New York Academy of Sciences·G Mazoujian, D E Haagensen
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P E Fielding, C J Fielding
Aug 1, 1995·Experimental Cell Research·Z ZhaoK J Soprano
Oct 11, 1994·Proceedings of the National Academy of Sciences of the United States of America·V CavaillèsM G Parker
Oct 15, 1993·Biochemical and Biophysical Research Communications·M GianniE Garattini
Jun 1, 1993·British Journal of Cancer·J F EliasonT Tsukaguchi

❮ Previous
Next ❯

Citations

Sep 5, 2002·Biochemical and Biophysical Research Communications·Ki-Hwan LeeMyung-Ju Ahn
Nov 4, 2000·Biochimica Et Biophysica Acta·T Bratt
Nov 4, 2000·Biochimica Et Biophysica Acta·E RassartR Milne
May 30, 2001·The Journal of Steroid Biochemistry and Molecular Biology·M KobayashiA Tanaka
Mar 30, 2001·European Journal of Biochemistry·H TschescheS Bartsch
Feb 1, 2011·International Journal of Biological Sciences·Hsiu-Hsia LinSin-Tak Chu
Feb 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Ann B Goodman, Arthur B Pardee
Aug 28, 1998·Leukemia & Lymphoma·T Koyama, M Shibakura
Sep 13, 2007·Molecular Carcinogenesis·Fadel S AlyaqoubMichael A Pereira
May 5, 2007·Biochimica Et Biophysica Acta·Sonia Do CarmoEric Rassart
May 9, 2015·Acta histochemica·Eva Martínez-PinillaJorge Tolivia
Aug 31, 2017·Scientific Reports·Aswin HariHsiao-Huei Chen
Jun 20, 2020·Gene·Eric RassartCatherine Mounier
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Tatarinov YuSD D Petrunin
Molecular Reproduction and Development
A V VieiraP M Vieira
© 2021 Meta ULC. All rights reserved