PMID: 9422346Jan 9, 1998Paper

Retinoic acid induces apoptosis-associated neural differentiation of a murine teratocarcinoma cell line

Journal of Neurochemistry
T HergetA Maelicke

Abstract

Incubation with all-trans retinoic acid (RA) induces PCC7-Mz1 embryonic carcinoma cells to cease proliferation and to develop into a tissue-like pattern of neuronal, astroglial, and fibroblast-like derivatives over a period of several days. Concomitant with the induction of differentiation by RA, a sizable fraction of the Mz1 stem cells detaches and dies, with the maximal level of cell death achieved after 10 h of RA treatment. This RA-induced cell death fulfills all criteria of apoptosis, including nuclear condensation, intranucleosomal DNA degradation, expression of cysteine aspases (caspases), and the formation of apoptotic bodies. Apoptosis could be suppressed by the pan-caspase inhibitor zVAD-fmk (benzyloxycarbonyl-valinyl-alaninyl-aspartyl fluoromethyl ketone). Induction of apoptosis required at least 2 h of incubation with RA and followed the same RA concentration (EC50 = 10(-7) M RA) and time dependence as the induction of differentiation as delineated by the expression of the neuron-specific protein kinase C substrate GAP-43. RA-induced apoptosis increased with the plating density of PCC7-Mz1 cells. This effect was not due to deprivation of an essential nutrient or factor from the medium because apoptosis was not signi...Continue Reading

Citations

Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xiu Jun WangC Roland Wolf
May 29, 2010·Development, Growth & Differentiation·Yang BiTingyu Li
Oct 19, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·P GunasekarG E Isom
Oct 22, 2002·The Journal of Pharmacology and Experimental Therapeutics·K PrabhakaranG E Isom
Apr 24, 2009·Journal of Cellular Physiology·Ding-Dar LeeMarjana Tomic-Canic
Jan 5, 2000·Journal of Neurochemistry·P S Sastry, K S Rao
Nov 12, 2009·Expert Review of Neurotherapeutics·Hyun-Pil LeeAlan Lerner
Jun 22, 2000·The Journal of Biological Chemistry·T HergetG van Echten-Deckert
Sep 23, 2008·Journal of Neuroscience Research·Hui ShenYun Wang
Jun 5, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Saber M Hussain, John M Frazier
Dec 2, 2004·Brain Research. Brain Research Reviews·Laura Colombaioni, Mercedes Garcia-Gil
Nov 11, 2005·Progress in Neurobiology·Peter McCaffery, Curtis K Deutsch

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