Retinoic acid induction of calcium channel expression in human NT2N neurons

Biochemical and Biophysical Research Communications
Z Y GaoB A Wolf

Abstract

Ca2+ channel expression and regulation of intracellular Ca2+ homeostasis were studied during retinoic acid (RA)-induced differentiation of the human teratocarcinoma cell line Ntera 2/C1.D1 (NT2- cells) into NT2N neurons, a unique model of human neurons in culture. The cytosolic Ca2+ level of undifferentiated NT2- cells was low (75 +/- 5 nM) and stable under basal conditions, and it was only marginally decreased (by 9%) upon removal of extracellular Ca2+. After 10 microM RA treatment, NT2- cells were irreversibly differentiated into a phenotype of neuron-like NT2N cells. Cytosolic Ca2+ level of NT2N neurons was higher (106 +/- 14 nM) than that of NT2- cells and spontaneously fluctuated (0.208 +/- 0.038 transients/min) under basal conditions. Although K+ increased 86Rb fluxes in both NT2- cells and NT2N neurons, it only increased cytosolic Ca2+ level in NT2N neurons. The K+-induced increase in cytosolic Ca2+ in NT2N neurons was antagonized by 0.1-10 microM nifedipine or verapamil, 5 microM omega-CgTx GVIA, but not by 1 microM omega-agatoxin IVA, 1 microM omega-agatoxin TK, 1 microM FTX-3.3, or 100 microM Ni+ implicating L- and N-type voltage-dependent Ca2+ channels. In L- and N-type channels, but not in P- and Q-types, mRNAs were...Continue Reading

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Citations

Aug 5, 2009·Neurochemistry International·Johanna El Andaloussi-LiljaAnna Forsby
Apr 22, 2006·Developmental Biology·Jennifer M DmetrichukGaynor E Spencer
Dec 29, 2013·Journal of Neurophysiology·Nicholas D Vesprini, Gaynor E Spencer
Oct 25, 2014·Journal of Neurophysiology·Nicholas D VespriniGaynor E Spencer
Dec 9, 2000·Journal of Neurophysiology·T R NeelandsR L Macdonald
May 11, 2005·Progress in Neurobiology·Michelle A Lane, Sarah J Bailey

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