Retinoic acid inhibits hydrocortisone-stimulated expression of phenol sulfotransferase in bovine bronchial epithelial cells

Journal of Cellular Physiology
J D BeckmannB Kliewer

Abstract

The airway epithelium, which is commonly exposed to xenobiotics, contains the conjugative enzyme phenol sulfotransferase (PST). We have previously reported that hydrocortisone (HC) stimulates the expression of PST severalfold in cultured bovine bronchial epithelial cells (Beckmann et al., 1994, J. Cell. Physiol. 160:603-610). Here we report that this stimulation is attenuated by retinoic acid (RA). Dose-response measurements of both enzyme activities and mRNA levels indicated a 50% inhibition of HC-stimulated PST expression with 0.05 nM RA. Varied concentrations of RA had a general repressive effect on HC-stimulated PST expression, with no change in the half-maximal HC stimulatory concentration of 12.5 nM. Steady state kinetic measurements indicated no significant changes in apparent Km values of 3-5 microM for the acceptor substrate, 2-naphthol; only HC- and RA-dependent changes in Vmax were observed. These changes were likely due to altered enzyme expression, as evidenced by immunoblot and Northern blot hybridization analyses. Thus, the expression of PST within bronchial epithelial cells is not merely constitutive, but is subject to both positive and negative controls.

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Citations

Oct 13, 2001·Journal of Cellular Physiology·J D BeckmannT P Keeton
May 10, 2005·Biochimica Et Biophysica Acta·Dongning HeCharles N Falany
Jan 26, 2005·Basic & Clinical Pharmacology & Toxicology·Smarajit MaitiGuangping Chen
Nov 28, 2006·Archives of Biochemistry and Biophysics·Sundari ChodavarapuJoe D Beckmann

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