Retinoic acid inhibits osteogenic differentiation of rat bone marrow stromal cells

Biochemical and Biophysical Research Communications
An-Xun WangZhaoyou Yao

Abstract

It has been well established that all-trans-retinoic acid (ATRA) influences bone metabolism when given in the treatment or prevention of cancer. However, the molecular mechanisms underlying this are unknown. In the present study, we investigated the effect of ATRA on differentiation of rat bone marrow stromal cells (BMSCs). BMSCs were harvested from rats and induced to differentiate in the presence or absence of ATRA in either osteogenic (OM) or control medium (CM). BMSCs underwent osteogenic differentiation, showed alkaline phosphatase (ALP) activity, a high level of matrix mineralization, and expressed osteonectin when cultured in OM. Although ATRA induced ALP activity, it failed to induce matrix mineralization and osteonectin, decrease mineralization in OM, and induce lipid accumulation in BMSCs. Moreover, while ATRA induced the expression of BMP-RIA, both BMP-RII and Smad5 mRNA were induced by OM and ATRA. Thus, ATRA inhibited osteogenesis and promoted adipogenesis of BMSCs. BMP signaling cooperated with ATRA in the differentiation of BMSCs.

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Citations

Mar 9, 2011·Journal of Bone and Mineral Metabolism·Wayne BalkanBruce R Troen
Oct 6, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Alanna C GreenLouise E Purton
Apr 13, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Zhaoyou YaoTingting Zhao
Nov 10, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Mu ChenAn-Xun Wang
Jan 24, 2015·Differentiation; Research in Biological Diversity·Yanhong YuMichal Opas
May 24, 2012·Biochemical and Biophysical Research Communications·Shuichi SakamotoYoshio Nishimura
May 31, 2013·Endocrine Reviews·H Herschel ConawayUlf H Lerner
Dec 20, 2019·Arteriosclerosis, Thrombosis, and Vascular Biology·Maximillian A RogersJorge Plutzky
Feb 28, 2019·Journal of Applied Oral Science : Revista FOB·Ariadne Cristiane Cabral CruzCláudia Maria Oliveira Simões
Jan 19, 2013·Journal of Applied Oral Science : Revista FOB·Ariadne Cristiane Cabral CruzCláudia Maria Oliveira Simões

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