Retinoic acid promotes in vitro follicle activation in the cat ovary by regulating expression of matrix metalloproteinase 9

PloS One
M FujiharaN Songsasen

Abstract

Retinoic acid (RA) facilitates tissue morphogenesis by regulating matrix matalloproteinase (MMPs) expression. Our objective was to examine the influence of RA on in vitro development of follicles enclosed within domestic cat ovarian tissues. Ovarian cortices from 9 prepubertal and 13 adult cats were incubated for 7 d in medium containing 0 (control), 1 or 5 μM RA and then analyzed for viability. Cortices from additional three animals of each age group were cultured in the same condition and follicle morphology, stage and size were histologically evaluated. In a separate study, cortices from 14 donors (7 prepubertal; 7 adult cats) were incubated in 0 or 5 μM RA for 7 d and assessed for (1) MMP1, 2, 3, 7, 9 and TIMP1 expression by qPCR and (2) protein expression of MMP9 by immunohistochemistry. Donor age did not influence follicle response to RA. Collective data from both age groups revealed that percentages of primordial follicles in 5 μM RA treatment were lower (P < 0.05; 40.5 ± 4.5%) than in fresh cortices (66.7 ± 5.3%) or controls (60.1 ± 4.0%) with 1 μM-RA treatment producing intermediate (56.3 ± 4.0%) results. Proportion of primary follicles in 5 μM RA (21.7 ± 3.3%) was higher than in fresh cortices (4.9 ± 2.9%) and control...Continue Reading

References

Jan 1, 1996·Biology of Reproduction·J J Eppig, M J O'Brien
Feb 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·A A MurrayN Spears
Oct 4, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Raymond J RodgersDarryl L Russell
Mar 8, 2005·The Journal of Reproduction and Development·Shuntaro IkedaMasayasu Yamada
Dec 29, 2005·Experimental Biology and Medicine·Masanori IiYasuhisa Shinomura
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·Andrea Page-McCawZena Werb
Aug 24, 2007·Development·Josephine Bowles, Peter Koopman
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Feb 12, 2013·Reproduction in Domestic Animals = Zuchthygiene·N SongsasenD E Wildt
Apr 29, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Linah Al-Alem, Thomas E Curry
Oct 13, 2015·Hormone Molecular Biology and Clinical Investigation·Sabine MeinelArtur Mayerhofer
Aug 4, 2016·Reproduction in Domestic Animals = Zuchthygiene·M FujiharaN Songsasen

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Methods Mentioned

BETA
light microscopy
PCR
reverse transcription PCR

Software Mentioned

IPLab
GraphPad Prism
Primer3

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