PMID: 9526050Apr 4, 1998Paper

Retinoic acid regulates gonadotropin-releasing hormone (GnRH) release and gene expression in the rat hypothalamic fragments and GT1-1 neuronal cells in vitro

Brain Research. Molecular Brain Research
S ChoK Kim

Abstract

The present study attempts to examine the possible involvement of retinoic acid (RA) in the regulation of gonadotropin-releasing hormone (GnRH) release and gene expression in the rat hypothalamic fragments and GT1-1 neuronal cells in vitro. During a short-term period (2h), RA (0.01-1 microM) increased GnRH release in a dose-related manner. Time-course experiments showed that RA rapidly increased GnRH release by 30 min in both cells. RA-induced GnRH release was slowly attenuated in the next incubation period in hypothalamic fragments, but rapidly returned to control levels in GT-1 cells. In hypothalamic fragments, GnRH mRNA levels decreased, but in GT1-1 cells, no change in GnRH mRNA levels was observed. We then extended the incubation time to see any changes in GnRH mRNA levels by RA in GT1-1 cells. In a long term (up to 48 h), RA increased GnRH mRNA levels in a dose- and time-related manner. Significant increase in GnRH mRNA levels by RA (at higher than 10 nM) was observed within 12h. Transient transfection experiments with a luciferase reporter vector containing more than 3 kb of the rat GnRH 5'-flanking region (-3002 to +88) revealed that RA also increased the rat GnRH promoter activity in a similar dose-and time-dependent m...Continue Reading

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Citations

Jun 2, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yura KobayashiShigeki Fujiwara
May 11, 2005·Progress in Neurobiology·Michelle A Lane, Sarah J Bailey
Oct 17, 2002·Frontiers in Neuroendocrinology·J Peter H Burbach
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Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Han Kyoung ChoeKyungjin Kim
Aug 24, 2012·Experimental & Molecular Medicine·Yeon Ju LeeSe Hyung Cho
Mar 8, 2014·Experimental & Molecular Medicine·Jong-Yun NohSehyung Cho
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Sep 25, 2009·Journal of Pediatric Endocrinology & Metabolism : JPEM·Zvi Zadik
Apr 27, 2017·The Journal of Veterinary Medical Science·Kiran PandeyHiroya Kadokawa

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