Retinoic acid regulates the expression of the calcium binding protein, calbindin-D28K

Molecular Endocrinology
Y Z Wang, S Christakos

Abstract

It is a well established fact that the calcium-binding protein, calbindin-D28k, is influenced by vitamin D in intestine and kidney. However, very little is known concerning the regulation of calbindin-D28k in brain. Although few genes that are regulated by retinoic acid (RA) have been identified in the nervous system, we now report that the human medulloblastoma cell line D283 (which is derived from cerebellum and has a distinctly neuronal phenotype) contains calbindin-D28k endogenously and that calbindin protein and mRNA can be induced 10- to 15-fold in these cells by 10(-7) M RA. These findings are the first evidence of RA-mediated regulation of calbindin. The time course of response, as determined by Northern blot analysis, indicated that the first significant increase in calbindin-D28k mRNA is at 12 h with a plateau of calbindin mRNA induction at 72 h after RA treatment. The induction of calbindin mRNA by RA was preceded by an induction of retinoic acid receptor-alpha mRNA and was accompanied by an induction of retinoid X receptor-alpha mRNA. Calbindin-D28k mRNA levels in D283 medulloblastoma cells as well as the induction of calbindin mRNA by RA were not significantly affected by 1,25-dihydroxyvitamin D3 treatment. Deletio...Continue Reading

Citations

Jun 27, 2002·Journal of the Association for Research in Otolaryngology : JARO·Hakim HielPaul A Fuchs
May 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Kien C LuuLois A Salamonsen
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May 23, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Alba BolañosSylvie Babajko
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