Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Stem Cells International
Jiangyi WangSonglin Wang

Abstract

Retinoic acid (RA) signal is involved in tooth development and osteogenic differentiation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) are one of the useful MSCs in tissue regeneration. However, the function of RA in osteo/odontogenic differentiation of DPSCs remains unclear. Here, we investigated the expression pattern of RA in miniature pig tooth germ and intervened in the RA signal during osteo/odontogenic differentiation of human DPSCs. Deciduous canine (DC) germs of miniature pigs were observed morphologically, and the expression patterns of RA were studied by in situ hybridization (ISH). Human DPSCs were isolated and cultured in osteogenic induction medium with or without RA or BMS 493, an inverse agonist of the pan-retinoic acid receptors (pan-RARs). Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. We found that the expression of RARβ and CRABP2 decreased during crown calcification of DCs of miniature pigs. Activation of RA signal in vitro inhibited ALP activities ...Continue Reading

References

Apr 1, 1990·Trends in Neurosciences·D Summerbell, M Maden
Dec 1, 1994·Archives of Oral Biology·J E Kronmiller, C S Beeman
Sep 1, 1994·Differentiation; Research in Biological Diversity·A Bloch-ZupanJ V Ruch
Feb 25, 2000·Journal of Endodontics·M OhishiT Nagata
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Oct 31, 2001·Archives of Oral Biology·B K BerkovitzA W Barrett
Jun 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lovisa HessleJosé Luis Millan
Feb 7, 2003·Current Drug Metabolism·Julie MarillGuy G Chabot
Jun 24, 2006·Journal of Cellular Biochemistry·Toshihisa Komori
Sep 21, 2007·Nature Reviews. Neuroscience·Malcolm Maden
Mar 13, 2008·Current Biology : CB·Gonçalo C Vilhais-Neto, Olivier Pourquié
Dec 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wook-Young BaekJung-Eun Kim
Sep 29, 2009·Journal of Dental Research·S ChenM MacDougall
May 7, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yann GibertVincent Laudet
Jul 17, 2012·The Journal of Biological Chemistry·Qinglin ZhuChunlin Qin
Sep 4, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pawat SeritrakulWilliam R Jackman
Dec 18, 2013·PloS One·Thomas LindHåkan Melhus
May 23, 2014·Journal of Tissue Engineering and Regenerative Medicine·Marco TatulloLisa J White
Oct 6, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Alanna C GreenLouise E Purton
Oct 25, 2016·Stem Cell Research·Jinlei XiZheqiong Yang
Nov 8, 2016·Stem Cells International·Athina Bakopoulou, Imad About
Nov 25, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Timur M AbashevLisa L Sandell
Jan 24, 2017·Frontiers in Physiology·Supawich MorkmuedKaren Niederreither
Feb 15, 2017·Stem Cells International·Carolina De FuscoGiovanni Messina
Jun 13, 2017·Connective Tissue Research·Guoqing LiZhipeng Fan
Dec 22, 2017·Brazilian Oral Research·Yanhong YanYumei Zhao
Feb 2, 2018·BioMed Research International·Lei HuSonglin Wang
Jun 26, 2018·Cytotechnology·Ibrahim Mortada, Rola Mortada
Nov 6, 2018·International Journal of Oral Science·Helena Ritchie

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Citations

Jan 19, 2021·Stem Cell Reviews and Reports·Cheng LiangWeidong Tian

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