Retinoic acid signaling plays a restrictive role in zebrafish primitive myelopoiesis.

PloS One
Dong LiangQing-Shun Zhao

Abstract

Retinoic acid (RA) is known to regulate definitive myelopoiesis but its role in vertebrate primitive myelopoiesis remains unclear. Here we report that zebrafish primitive myelopoiesis is restricted by RA in a dose dependent manner mainly before 11 hpf (hours post fertilization) when anterior hemangioblasts are initiated to form. RA treatment significantly reduces expressions of anterior hemangioblast markers scl, lmo2, gata2 and etsrp in the rostral end of ALPM (anterior lateral plate mesoderm) of the embryos. The result indicates that RA restricts primitive myelopoiesis by suppressing formation of anterior hemangioblasts. Analyses of ALPM formation suggest that the defective primitive myelopoiesis resulting from RA treatment before late gastrulation may be secondary to global loss of cells for ALPM fate whereas the developmental defect resulting from RA treatment during 10-11 hpf should be due to ALPM patterning shift. Overexpressions of scl and lmo2 partially rescue the block of primitive myelopoiesis in the embryos treated with 250 nM RA during 10-11 hpf, suggesting RA acts upstream of scl to control primitive myelopoiesis. However, the RA treatment blocks the increased primitive myelopoiesis caused by overexpressing gata4/6...Continue Reading

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Citations

Jan 23, 2014·The International Journal of Biochemistry & Cell Biology·Zhenhong SuQing K Wang
Jun 15, 2014·Biochimica Et Biophysica Acta·Eric SamarutYann Gibert
Aug 27, 2014·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jingyun LiQingshun Zhao
May 3, 2013·The Journal of Clinical Investigation·Efterpi KostareliJochen Hess
Nov 20, 2016·EMBO Molecular Medicine·Antonino MontalbanoGudrun A Rappold
Aug 8, 2021·Nanomaterials·Alba Pensado-LópezFernando Torres Andón

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