Retinoic acid signaling targets Hox genes during the amphioxus gastrula stage: insights into early anterior-posterior patterning of the chordate body plan

Developmental Biology
Demian KoopMichael Schubert

Abstract

Previous studies of vertebrate development have shown that retinoic acid (RA) signaling at the gastrula stage strongly influences anterior-posterior (A-P) patterning of the neurula and later stages. However, much less is known about the more immediate effects of RA signaling on gene transcription and developmental patterning at the gastrula stage. To investigate the targets of RA signaling during the gastrula stage, we used the basal chordate amphioxus, in which gastrulation involves very minimal tissue movements. First, we determined the effect of altered RA signaling on expression of 42 genes (encoding transcription factors and components of major signaling cascades) known to be expressed in restricted domains along the A-P axis during the gastrula and early neurula stage. Of these 42 genes, the expression domains during gastrulation of only four (Hox1, Hox3, HNF3-1 and Wnt3) were spatially altered by exposure of the embryos to excess RA or to the RA antagonist BMS009. Moreover, blocking protein synthesis with puromycin before adding RA or BMS009 showed that only three of these genes (Hox1, Hox3 and HNF3-1) are direct RA targets at the gastrula stage. From these results we conclude that in the amphioxus gastrula RA signaling ...Continue Reading

References

Aug 1, 1993·The Journal of Steroid Biochemistry and Molecular Biology·F Rudert, H Gronemeyer
Jul 2, 1999·Experimental Cell Research·A JacobR R Reichel
Aug 24, 1999·Developmental Biology·H WadaP W Holland
Dec 23, 1999·Current Biology : CB·V EaswaranS Byers
Mar 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M SchubertN D Holland
Jun 22, 2000·Genesis : the Journal of Genetics and Development·M SchubertN D Holland
Feb 17, 2001·Development Genes and Evolution·L Z HollandM Schubert
Oct 29, 2002·Journal of Lipid Research·James E Balmer, Rune Blomhoff
Jan 7, 2003·The EMBO Journal·Tony OosterveenJacqueline Deschamps
Jun 12, 2003·Gene Expression Patterns : GEP·Kan ichiro Nagatomo, Shigeki Fujiwara
Jun 28, 2003·Development, Growth & Differentiation·Tomoko IshibashiShigeki Fujiwara
Jul 2, 2003·Development·Sylvie ForlaniJacqueline Deschamps
Aug 13, 2003·The European Journal of Neuroscience·P J McCafferyE Rosa-Molinar
Jul 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Michael SchubertVincent Laudet
Dec 4, 2004·Methods in Cell Biology·Linda Z Holland, Ju-Ka Yu
Feb 11, 2005·Molecular Biology and Evolution·Hervé PhilippeHenner Brinkmann
Mar 1, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shigeki Fujiwara
May 21, 2005·The International Journal of Developmental Biology·Anne Grapin-Botton
Jun 10, 2005·Development·Jacqueline Deschamps, Johan van Nes
Aug 17, 2005·Developmental Biology·Lisa Maves, Charles B Kimmel
Dec 13, 2005·Developmental Biology·Wataru KimuraKimiko Fukuda
Mar 16, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Hiroshi WadaVincent Laudet
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
May 12, 2006·Journal of Neurobiology·Joel C GloverFilippo M Rijli
May 31, 2006·International Journal of Biological Sciences·Ferdinand MarlétazMichael Schubert
Dec 23, 2006·Developmental Biology·Aida HalilagicMichèle Studer
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Chiharu Kimura-YoshidaIsao Matsuo
Apr 3, 2007·Developmental Biology·Cristian Cañestro, John H Postlethwait
Jul 20, 2007·Differentiation; Research in Biological Diversity·Nigel P Mongan, Lorraine J Gudas
Aug 21, 2007·Mechanisms of Development·Hans J JansenAntony J Durston
Sep 18, 2007·Current Opinion in Genetics & Development·Jacqueline Deschamps

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Citations

Aug 23, 2011·Genome Biology and Evolution·Ricard AlbalatMichael Schubert
Nov 17, 2011·BMC Evolutionary Biology·Alfonso NataleAnnamaria Locascio
Feb 7, 2012·Briefings in Functional Genomics·Yu-Bin WangJr-Kai Yu
Mar 24, 2012·Briefings in Functional Genomics·Claire LecroiseyMichael Schubert
Jun 23, 2011·Seminars in Cell & Developmental Biology·Juliana Gutierrez-MazariegosMichael Schubert
Mar 1, 2012·Wiley Interdisciplinary Reviews. Developmental Biology·Linda Z Holland, Takayuki Onai
Aug 14, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Grace S LeeMichael D Collins
Feb 5, 2014·Developmental Biology·Gerhard SchlosserSebastian M Shimeld
May 3, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Rafael Guerrero-PrestonDavid Sidransky
Dec 3, 2014·Chemico-biological Interactions·Olga V BelyaevaNatalia Y Kedishvili
Jul 14, 2016·Molecular Biology and Evolution·Josep Martí-SolansCristian Cañestro
Feb 2, 2018·Cellular and Molecular Life Sciences : CMLS·Elisabeth ZiegerMichael Schubert
Jan 14, 2019·Molecular Genetics & Genomic Medicine·Chao FangKai-Ming Duan
Apr 26, 2019·Genesis : the Journal of Genetics and Development·Antony J Durston
May 22, 2019·Genesis : the Journal of Genetics and Development·Christof NolteRobb Krumlauf
Aug 5, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Hugo J Parker, Robb Krumlauf
Jan 14, 2018·Genesis : the Journal of Genetics and Development·Aditi DubeyJean-Pierre Saint-Jeannet
Aug 12, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Karen D CrowNoelle A Kaslly
Sep 21, 2018·Frontiers in Cell and Developmental Biology·Antony J Durston

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