Retinoic acid stability in stem cell cultures

The International Journal of Developmental Biology
Kyle A SharowMary Ann Asson-Batres

Abstract

It has been reported that retinoids, such as retinoic acid (RA) and retinol (ROL), dissolved in aqueous solutions are susceptible to oxidative damage when exposed to light, air, and relatively high temperatures, conditions that are normal for culturing stem cells. Thus, questions arise regarding the interpretation of results obtained from studies of mouse embryonic stem cells exposed to retinoids because their isomerization state, their stability in culture conditions, and their interactions with other potential differentiation factors in growth media could influence developmental processes under study. Media samples were supplemented with retinoids and exposed to cell culture conditions with and without mouse embryonic stem cells (mESC), and retinoids were extracted and analyzed using HPLC. To determine whether retinoids are stable in media supplemented with fetal bovine serum (FBS) or in chemically-defined, serum-free media, mESC adapted to each type of growth media were investigated. Studies reported here indicate there was little loss or isomerization of at-RA, 9-cis-RA, 13-cis-RA, or ROL in cell cultures grown in serum-supplemented media when cell cultures were maintained in the dark and manipulated and observed under yell...Continue Reading

Citations

Jun 6, 2013·Stem Cells and Development·Barbara OrsolitsZsuzsanna Környei
Oct 31, 2015·Methods : a Companion to Methods in Enzymology·Roman Korytnikov, Maria Cristina Nostro
May 10, 2017·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Amir MinoviHeike Conrad
Nov 5, 2019·Cellular and Molecular Life Sciences : CMLS·Carlos Godoy-ParejoGuokai Chen

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