Retinoic acid stimulates transcription of the rat SHARP-2 gene via multiple pathways.

Journal of Biochemistry
Momoko HayashiKazuya Yamada

Abstract

Members of the enhancer of split- and hairy-related protein (SHARP) family, SHARP-1 and SHARP-2, are basic helix-loop-helix transcriptional repressors and belong to the clock genes. In this study, an effect of retinoic acid (RA) on the SHARP family gene expression in the differentiated cells was examined. RA rapidly and temporarily induced the SHARP-2 mRNA expression in hepatic H4IIE cells. Then, whether the SHARP-2 mRNA expression is altered by dexamethasone (Dex), insulin, and the combination of RA and Dex or RA and insulin was examined. Dex had different effects on the expression of SHARP-2 mRNA in the presence or absence of RA. Then, the molecular mechanisms were investigated using inhibitors of various signaling molecules. The RA-induction of SHARP-2 mRNA level was mainly inhibited by LY294002, staurosporine, and actinomycin D, respectively. Finally, whether RA acts on the transcriptional regulatory region of the SHARP-2 gene was analysed using luciferase reporter gene assay. At least two RA-responsive regions were mapped at the nucleotide sequences between -3,700 and -1,600 of the SHARP-2 gene. In addition, this effect was dependent on the RA receptor and retinoid X receptor. Thus, we conclude that RA stimulated transcrip...Continue Reading

References

Jul 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·G J Fisher, J J Voorhees
Mar 21, 2002·Nature Reviews. Cancer·L Altucci, H Gronemeyer
Oct 29, 2002·Journal of Lipid Research·James E Balmer, Rune Blomhoff
Jun 11, 2003·The Journal of Biological Chemistry·Kazuya YamadaKaoru Miyamoto
Oct 29, 2003·The Journal of Biological Chemistry·Aline Grechez-CassiauFranck Delaunay
Jan 17, 2004·Archives of Biochemistry and Biophysics·Satoko HiranoKaoru Miyamoto
Jun 23, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Kazuya Yamada, Kaoru Miyamoto
Nov 4, 2005·Journal of Biological Rhythms·Mitsuhide NoshiroYukio Kato
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Takahiro NakamuraKunio Matsumoto
Jun 12, 2012·Archives of Biochemistry and Biophysics·Ayumi HaneishiKazuya Yamada
Feb 27, 2013·Journal of Lipid Research·Ziad Al TanouryCécile Rochette-Egly
Jan 22, 2014·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·K TakagiK Yamada
Mar 25, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mathilde Raverdeau, Kingston H G Mills
Jul 29, 2015·Advances in Experimental Medicine and Biology·Daryl K GrannerKeith R Yamamoto
Aug 10, 2015·Developmental Biology·Jonathan T BusadaChristopher B Geyer
Jan 29, 2016·Journal of Cancer·Fuyuki SatoYasuteru Muragaki
Oct 30, 2016·Bioscience, Biotechnology, and Biochemistry·Yukiko KanaiKazuya Yamada

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.