Retinoic acids increase P2X2 receptor expression through the 5'-flanking region of P2rx2 gene in rat phaeochromocytoma PC-12 cells

Molecular Pharmacology
Hidetoshi Tozaki-SaitohKazuhide Inoue

Abstract

The P2X2 receptor is a subtype of ionotropic ATP receptor and plays a significant role in regulating fast synaptic transmission in the nervous system. Because the expression level of the P2X2 receptor is known to determine its channel properties and functional interactions with other neurotransmitter channels, elucidating the mechanisms underlying the regulation of P2X2 receptor expression in neuronal cells is important. Here, we identified three motifs that correspond to the retinoic acid response element in the 5'-flanking region of the rat P2X2 gene. In rat pheochromocytoma PC-12 cells, treatment with 9-cis-retinoic acid as well as all-trans-retinoic acid significantly increased the mRNA and protein level of P2X2 receptor. In addition, in PC-12 cells transiently transfected with a luciferase reporter gene driven by the promoter region of the rat P2X2 gene, both 9-cis-retinoic acid and all-trans-retinoic acid increased the luciferase activity, whereas their effects were diminished by truncation of the retinoic acid response elements in the promoter. Furthermore, 9-cis-retinoic acid enhanced the ATP-evoked whole cell currents and intracellular Ca2+- and ATP-evoked dopamine release, indicating the up-regulation of functional P2...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P N MacDonaldD E Ong
Aug 1, 1993·The Journal of Cell Biology·A Batistatou, L A Greene
Jan 1, 1995·Journal of Neuroscience Research·I R Boniece, J A Wagner
Mar 1, 1993·Journal of Cellular Physiology·S RahaJ K Reed
Jan 19, 2000·Progress in Neurobiology·S A Waterman
Jun 28, 2001·Physiological Reviews·A Aranda, A Pascual
Dec 18, 2001·American Journal of Physiology. Cell Physiology·George I Gorodeski
Jul 26, 2002·Journal of Medicinal Chemistry·Bitoku TakahashiHiroyuki Kagechika
Sep 25, 2002·Physiological Reviews·R Alan North
Apr 4, 2003·Cancer Biology & Therapy·Karen LibyNira Ben-Jonathan
Jan 8, 2004·European Journal of Pharmacology·Peter Illes, J Alexandre Ribeiro
Mar 27, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Eiji Shigetomi, Fusao Kato
Apr 20, 2004·Neuroreport·Yue WangKatsutoshi Furukawa
Apr 27, 2004·The Journal of Physiology·Yuichiro Fujiwara, Yoshihiro Kubo
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo J RodriguesRodrigo A Cunha

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Citations

Nov 3, 2009·Molecular and Cellular Biochemistry·Elsie A OrellanoFernando A González
Jun 26, 2013·Purinergic Signalling·Geoffrey Burnstock, Francesco Di Virgilio
Oct 17, 2007·American Journal of Medical Genetics. Part a·Dmitriy M NiyazovMargaret P Adam
Nov 19, 2009·American Journal of Medical Genetics. Part a·Irene Mademont-SolerAnna Soler
Aug 14, 2013·The British Journal of Dermatology·M Sárdy, T Ruzicka
Sep 12, 2009·Critical Reviews in Toxicology·Helmut GreimHeinz-Walter Thielmann

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