Retinoid-X receptors and the effects of 9-cis-retinoic acid on insulin secretion from RINm5F cells

Metabolism: Clinical and Experimental
B S ChertowK A Matthews

Abstract

Retinoid-X receptors (RXRs) are 9-cis-retinoic acid (9CRA)-dependent gene transcription factors, which modulate the action of all-trans-retinoic acid (ATRA), fatty acids, thyroid hormone (TH), and vitamin D (VD) by forming dimers with themselves or ATRA, TH, peroxisome proliferator activator receptors (PPARs), or VD receptors (VDRs). To determine if 9CRA and RXRs have a role in secretion, RINm5F cells were assayed for RXR transcripts and effects of 9CRA and ATRA on secretion. A single RXR alpha transcript and two RXR beta transcripts, but not RXR gamma, were evident by Northern blot. Cells were cultured for 48 hours without and with 9CRA 1 to 1,000 nmol/L and then stimulated with glucose 0, 0.5, 2.8, 7, and 11 mmol/L 9CRA increased secretion at each glucose concentration, 9CRA increased secretion by 50% to 100% (ANOVA, P < .001) with consistent concentration-dependent responses (eg. at glucose 2.8 mmol/L 9CRA: 0 nmol/L, 5.02 +/- .20 ng/(10(6) cells.h); 1 nmol/L, 6.97 +/- .30; 10 nmol/L, 8.36 +/- .18; 100 nmol/L, 9.15 +/- .28; 1,000 nmol/L, 10.24 +/- .24; n = 6). Although RINm5F cells respond slightly if at all to glucose, 9CRA facilitated glucose-induced insulin release (eg, at 9CRA 100 nmol/L, glucose: 0.5 mmol/L, 7.47 +/- .22...Continue Reading

Citations

Jul 31, 2004·The Journal of Nutrition·Kimberly A MatthewsBruce S Chertow
Jan 3, 2013·BioFactors·Pierre-Jacques BrunWilliam S Blaner
Jun 28, 2012·Diabetes & Metabolism Journal·Eun-Jung Rhee, Jorge Plutzky
Aug 22, 2015·Current Diabetes Reports·William T MooreDongmin Liu
Jan 25, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Ioan Ovidiu SirbuAlexander Radu Moise
Dec 6, 2001·Diabetes Research and Clinical Practice·C D BerdanierC E Mathews
Dec 9, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Enmin Zou, Ryan Bonvillain
Oct 30, 2007·General and Comparative Endocrinology·P Sreenivasula Reddy, S B Sainath
Oct 11, 2011·Biochimica Et Biophysica Acta·Maureen A Kane

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