Retinol-binding protein: the transport protein for vitamin A in human plasma

The Journal of Clinical Investigation
M KanaiD S Goodman

Abstract

Vitamin A circulates in human plasma as retinol bound to a specific transport protein. This protein differs from the known low and high density plasma lipoproteins and has a hydrated density greater than 1.21. In order to study this protein, volunteers were injected intravenously with retinol-15-(14)C. Plasma was collected 1-3 days later, and the purification of retinol-binding protein (RBP) was monitored by assaying for (14)C and also by following the fluorescence of the protein-bound retinol. Purification of RBP was effected by the sequence: Cohn fractionation, chromatography on columns of Sephadex G-200 and diethylaminoethyl (DEAE)-Sephadex, preparative polyacrylamide gel electrophoresis, and finally chromatography on Sephadex G-100. These procedures resulted in a preparation of RBP which was at least 98% pure and which had been purified more than 1500-fold. Purified RBP has alpha(1) mobility on electrophoresis and has a molecular weight of approximately 21,000-22,000. There appears to be one binding site for retinol per molecule of RBP. Solutions of RBP are fluorescent (characteristic of retinol) and have ultraviolet absorption spectra with peaks at 330 mmu (resulting from the bound retinol) and at 280 mmu. There are no fat...Continue Reading

References

Apr 18, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·C C BlakeV R Sarma
Jan 5, 1967·The New England Journal of Medicine·D S FredricksonR S Lees
Mar 4, 1967·Nature·G KarthaD Harker
Sep 1, 1955·The Journal of Clinical Investigation·R J HAVELJ H BRAGDON
Jan 1, 1958·Archives of Biochemistry and Biophysics·N I KRINSKYJ L ONCLEY
Jan 1, 1960·Scandinavian Journal of Clinical and Laboratory Investigation·H J MULLER-EBERHARD
Nov 27, 1962·Biochemical and Biophysical Research Communications·V SHORE, B SHORE
Mar 29, 1963·Science·J C KENDREW
Dec 1, 1964·The Journal of Clinical Investigation·K SCHMIDH YOSHIZAKI
Nov 1, 1964·Analytical Biochemistry·T JOVINM A NAUGHTON
Mar 1, 1965·The Journal of Clinical Investigation·R I LEVY, D S FREDRICKSON
Dec 1, 1964·Experimental Eye Research·J GLOVER, R J WALKER
Aug 1, 1962·The Journal of Clinical Investigation·A SCANU, W L HUGHES

❮ Previous
Next ❯

Citations

Jan 29, 2002·Analytical Biochemistry·Urban A KiernanRandall W Nelson
Jan 1, 1984·Archives of Dermatological Research·H Törmä, A Vahlquist
Jan 1, 1983·Archives of Dermatological Research·H Törmä, A Vahlquist
Apr 1, 1994·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·G C SmithM Guy
Nov 1, 1992·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·P A Tomlinson
Jun 1, 1996·Human Genetics·X N ChenH K Fong
Sep 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M MiyazakiJ Sato
May 4, 2013·Cell and Tissue Research·Alexander R OrtloffEsteban Rodríguez
Oct 3, 1986·Biochimica Et Biophysica Acta·G van DesselW Dierick
Sep 8, 1993·Biochimica Et Biophysica Acta·A V Vieira, W J Schneider
Jul 21, 1972·Biochimica Et Biophysica Acta·A M GottoD S Goodman
Aug 18, 1975·Clinica Chimica Acta; International Journal of Clinical Chemistry·Y IngenbleekM De Visscher
Apr 15, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·L ScarpioniC Buzio
Nov 24, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·A M BernardR Lauwerys
Apr 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·F GulamaliP A Pleban
Jan 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·A BernardR Lauwerys
Aug 31, 1992·Clinica Chimica Acta; International Journal of Clinical Chemistry·A W HodsonN B Argent
May 1, 1974·Journal of Insect Physiology·R P BodnarykJ K Koeppe
Mar 17, 1980·Life Sciences·L Barr-Nea, N Kariv
Jan 1, 1978·Progress in Lipid Research·F P Bell
Jan 1, 1981·Pharmacology & Therapeutics·D Tsambaos, C E Orfanos
Oct 1, 1989·Journal of Biochemical and Biophysical Methods·J M Chapman, R W Curley
Jun 5, 1980·Biochimica Et Biophysica Acta·E R BermanL Feeney-Burns
Jun 20, 1990·Biochimica Et Biophysica Acta·Y KandaY Yoshino
Jan 5, 1994·Biochimica Et Biophysica Acta·T J SmithM Schoenl
Aug 17, 1995·Biochimica Et Biophysica Acta·A JaworowskiR C Augusteyn
May 15, 1972·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C C HuangB D Owen
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R Berni, V Lamberti
Mar 1, 1992·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·W Duan, G Schreiber
May 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·A R AldredG Schreiber
May 1, 1990·The Journal of Nutritional Biochemistry·M E Davila-BloomD S Goodman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.