Retinophilin is a light-regulated phosphoprotein required to suppress photoreceptor dark noise in Drosophila.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Kirk L MecklenburgJoseph E O'Tousa

Abstract

Photoreceptor cells achieve high sensitivity, reliably detecting single photons, while limiting the spontaneous activation events responsible for dark noise. We used proteomic, genetic, and electrophysiological approaches to characterize Retinophilin (RTP) (CG10233) in Drosophila photoreceptors and establish its involvement in dark-noise suppression. RTP possesses membrane occupation and recognition nexus (MORN) motifs, a structure shared with mammalian junctophilins and other membrane-associated proteins found within excitable cells. We show the MORN repeats, and both the N- and C-terminal domains, are required for RTP localization in the microvillar light-gathering organelle, the rhabdomere. RTP exists in multiple phosphorylated isoforms under dark conditions and is dephosphorylated by light exposure. An RTP deletion mutant exhibits a high rate of spontaneous membrane depolarization events in dark conditions but retains the normal kinetics of the light response. Photoreceptors lacking neither inactivation nor afterpotential C (NINAC) myosin III, a motor protein/kinase, also display a similar dark-noise phenotype as the RTP deletion. We show that NINAC mutants are depleted for RTP. These results suggest the increase in dark no...Continue Reading

Citations

Jul 1, 2011·Proceedings of the National Academy of Sciences of the United States of America·Samuel S C RundGiles E Duffield
Jul 17, 2012·Journal of Neurogenetics·Karen L Hibbard, Joseph E O'Tousa
Jun 1, 2019·Scientific Reports·Bénédicte GnangnonChristine Pierrot
Mar 11, 2010·The Journal of Biological Chemistry·Olaf VoolstraArmin Huber

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