Retinoylation of the type II cAMP-binding regulatory subunit of cAMP-dependent protein kinase is increased in psoriatic human fibroblasts

Journal of Cellular Physiology
S TournierD Evain-Brion

Abstract

Previously, we have reported a defect in the cAMP-dependent protein kinases (cAMP-PK) in psoriatic cells (i.e., a decrease in 8-azido-[32P]cAMP binding to the regulatory subunits and a decrease in phosphotransferase activity) which is rapidly reversed with retinoic acid (RA) treatment of these cells. This led us to examine a possible direct interaction between retinoids and the RI and RII regulatory subunits through retinoylation. Retinoylation of RI and RII present in normal and psoriatic human fibroblasts was analysed by [3H]RA treatment of these cells, followed either by chromatographic separation of the regulatory subunits or by their specific immunoprecipitation. These studies indicated that RI and RII can be retinoylated. [3H]RA labeling of the RII subunit was significantly (P < 0.005) greater in psoriatic fibroblasts (nine subjects; mean 7.47 relative units +/- 1.37 SEM) compared to normal fibroblasts (eight subjects; mean 2.46 relative +/- 0.49 SEM). [3H]RA labeling of and the increase in 8-azido-[32P]-binding to the RI and RII subunit in psoriatic fibroblasts showed a similar time course. This suggests that the rapid effect of retinoic acid treatment to enhance 8-azido-[32P]-cAMP binding to the RI and RII in psoriatic ...Continue Reading

References

May 1, 1992·The Journal of Investigative Dermatology·J T ElderJ J Voorhees
Apr 1, 1991·The Journal of Investigative Dermatology·J T ElderJ J Voorhees
Jan 1, 1990·Methods in Enzymology·N Takahashi, T R Breitman
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A KrustP Chambon
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G W VasiosL J Gudas
May 27, 1989·Lancet·F RaynaudD Evain-Brion
Sep 14, 1989·Biochimica Et Biophysica Acta·B Renstrom, H F DeLuca
Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D E BrionW Anderson
Jan 1, 1987·Annual Review of Nutrition·F Chytil, D E Ong
Mar 1, 1973·Archives of Biochemistry and Biophysics·P BöhlenS Udenfriend
Jan 1, 1983·Methods in Enzymology·R Roskoski

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Citations

Mar 10, 2001·Biochimica Et Biophysica Acta·G Genchi, J A Olson
Nov 4, 2008·Biochimica Et Biophysica Acta·E CioneG Genchi
Jan 3, 2013·BioFactors·Pierre-Jacques BrunWilliam S Blaner
Dec 30, 2014·Molecular Aspects of Medicine·Alessandra di MasiClara Nervi
Jun 13, 2009·Journal of Bioenergetics and Biomembranes·Attilio PingitoreGiuseppe Genchi
May 12, 2006·Journal of Neurobiology·Rune Blomhoff, Heidi Kiil Blomhoff
May 22, 2010·Journal of Bioenergetics and Biomembranes·Valentina SenatoreGiuseppe Genchi
Sep 1, 2005·Molecular and Cellular Biochemistry·Erika CioneGiuseppe Genchi
May 21, 2005·Journal of Bioenergetics and Biomembranes·Erika CioneGiuseppe Genchi

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